부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1537-2995.2007.01169.x Byline: Miguel Lozano, Ana Galan, Roberto Mazzara, Laurence Corash, Gines Escolar Abstract: BACKGROUND: Amotosalen plus ultraviolet A light photochemical treatment (PCT) inactivates high titers of bacteria, and other pathogens, in platelet concentrates (PCs) potentially allowing the storage of platelets (PLTs) for up to 7 days. Adhesion and aggregation of PLTs to injured vascular surfaces are critical aspects of PLT hemostatic function. STUDY DESIGN AND METHODS: Two ABO-identical leukoreduced buffy coat-derived PCs in additive solution were mixed and divided: one-half underwent PCT (PCT-PCs) and the other was kept as a control (C-PCs); both were stored under standard conditions. The total number of paired PCs studied was nine. Samples were taken on Day 1 (before PCT) and after 5 and 7 days of storage. The adhesion and aggregation capacities were evaluated under flow conditions in a ex vivo perfusion model. RESULTS: Compared to control, PCT resulted in a decrease in PLT count of 6.5 percent (p = 0.004) and 10.2 percent (p = 0.008) after 5 and 7 days' storage, respectively (n = 9). PLT interaction with subendothelium was mainly in form of adhesion. The surface covered by PCT PLTs on Day 1 was 26.0 [+ or -] 4.2 percent (mean [+ or -] SEM). On Day 5, PCT-PCs showed a covered surface of 20.9 [+ or -] 2.2 percent, and the C-PCs, 20.6 [+ or -] 1.6 percent. After 7 days, PCT-PCs produced a nonsignificant higher PLT deposition compared to control (27.1 [+ or -] 2.9% vs. 21.2 [+ or -] 2.8%, p = 0.06). CONCLUSION: PCT of PCs and storage up to 7 days was associated with a 10.2 percent decrease in PLT count due to processing losses compared to C-PC. PLT adhesive and aggregating capacities under flow conditions of PCT-PCs were similar to C-PCs and remained well preserved for up to 7 days of storage. Article History: Received for publication July 19, 2006; revision received September 27, 2006, and accepted October 3, 2006. Article note: Dr Miguel Lozano, Hospital Clinic Provincial, Department of Hemotherapy and Hemostasis, Villarroel 170, 08036 Barcelona, Spain; e-mail: mlozano@clinic.ub.es.