부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.biologicals.2005.11.004 Byline: Hsiaoling Wang, Alfred V. Del Grosso, Joan C. May Abstract: A novel and sensitive HPLC method for the determination of benzethonium chloride (BZC) in anthrax vaccine was developed. Adjuvant Alhydrogel was removed by syringe filter after a simple sample pretreatment -- acidification prior to injection. Chromatography was performed by isocratic reverse phase separation with methanol/262mM ammonium acetate (80/20, v/v) on an endcapped C18 column with diode array detector (DAD). The method showed excellent recovery (100[+ or -]1.5%). The results indicated that this method could accurately determine BZC at the limit of detection (LOD) of 0.5ppm and the limit of quantitation (LOQ) of 1.5ppm with dynamic range up to 100ppm. The comparison of analysis between new HPLC and old titrimetric methods is also reported. The HPLC method is proven to be more accurate and precise with much less vaccine sample and human labor required. Author Affiliation: Laboratory of Analytical Chemistry, Office of Vaccine Research and Review, Center of Biological Evaluation and Research, Food and Drug Administration, 1401 Rockville Pike HFM-406, Rockville, MD 20852, USA Article History: Received 16 November 2005; Accepted 18 November 2005