부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.gene.2005.05.031 Byline: Kiyoshi Matsubara (a), Hiroaki Kodama (b), Hisashi Kokubun (a), Hitoshi Watanabe (c), Toshio Ando (b) Keywords: Anthocyanidin; Flavonoid-3',5'-hydroxylase; Petunia; Retrotransposon; Transposon Abbreviations: F3'5'H, Flavonoid-3',5'-hydroxylase; LTR, Long terminal direct repeat; ORF, Open reading frame; RTase, Reverse transcriptase; kbp, Kilobase pairs; cDNA, DNA complementary to RNA Abstract: The gene Hf1 plays a key role in the expression of floral color in petunias. Hf1 encodes a flavonoid-3',5'-hydroxylase (F3'5'H). The recessive allele (hf1) in an inbred line of petunia is known to be generated by the insertion of a transposable element (Psl). We isolated a novel Mutator-like transposable element (named dTph9) from the hf1 allele of a commercial petunia with red flowers. Another novel transposable element (named rTph1) was found in hf1 of another red petunia. rTph1 shared features with the copia-like retrotransposable element family. These novel elements were inserted independently in the third exon of the Hf1 gene, at different positions. The hf1 allele harboring dTph9 or rTph1 cannot produce an active F3'5'H enzyme because there are stop codons in the dTph9 and rTph1 sequences. Southern analysis showed that these elements were present in relatively low copy numbers and that mutation of the Hf1 locus was associated with the transposition of both elements. We conclude that a loss-of-function mutation of the petunia Hf1 gene is caused by the insertion of at least two different transposable elements, other than Psl, within the Hf1 gene. Author Affiliation: (a) Graduate School of Science and Technology, Chiba University, 1-33 Yayoi-cho, Inage-ku, Chiba 263-8522, Japan (b) Faculty of Horticulture, Chiba University, 648 Matsudo, Chiba 271-8510, Japan (c) Center of Environment, Health and Field Science, Chiba University, 6-2-1, Kashiwanoha, Kashiwa, Chiba 277-0882, Japan Article History: Received 7 April 2005; Revised 5 May 2005; Accepted 16 May 2005 Article Note: (miscellaneous) Received by S. Yokoyama