부산대학교 도서관

Purpose To characterize Escherichia coli ventilator-associated pneumonia (VAP) in intensive care unit (ICU) patients by determining antibioresistance and genotypic characteristics of E. coli isolates responsible for VAP or lung colonization, by comparing them with their oropharyngeal and rectal counterparts and by assessing representative isolates' virulence in a pneumonia mouse model. Methods Patients under mechanical ventilation for more than 72 h were screened for simultaneous presence of E. coli in rectal, oropharyngeal, and respiratory samples (colonization or VAP). If present, E. coli isolates were characterized by antimicrobial susceptibility, phylogenetic grouping, and virulence factor (VF) gene content determination. BALB/c mice were challenged intranasally with 3.6 x 10.sup.8 colony-forming units (CFU) of patients' E. coli isolates. Results Multisite E. coli colonization was observed in 19 % of patients (25 patients, 12 with E. coli VAP). One hundred fifteen distinct E. coli isolates were analyzed. B2 phylogenetic group was predominant, with high VF gene content and low antimicrobial resistance. Antimicrobial resistance diversity was observed in four patients with VAP. E. coli isolates from VAP patients were more frequently B2 isolates, with significantly greater VF gene content than lung colonization isolates. Among screened VF genes, iroN and sfa appeared important for lung infection. A very strong correlation (R.sup.2 = 0.99) was found between VF gene content and mortality in the mouse model. Conclusions This is the first study establishing antibioresistance and genotypic characteristics of E. coli isolates responsible for VAP in adult ICU patients. These isolates are highly virulent specific extraintestinal pathogenic E. coli strains expressing virulence factors, representing potential targets for new therapies.
Author(s): Jonathan Messika [sup.1] [sup.2] [sup.3], Fatma Magdoud [sup.4], Olivier Clermont [sup.1] [sup.2], Dimitri Margetis [sup.1] [sup.2] [sup.3], Stéphane Gaudry [sup.1] [sup.2] [sup.3], Damien Roux [sup.1] [sup.2] [sup.3], Catherine Branger [...]