학술논문
The effect of complex herbal extract and methotrexate on suppressing adjuvant arthritis in rats/Ucinak mjeavine biljnih ekstrakata i metotreksata na supresiju adjuvantnog artritisa u takora
Document Type
Academic Journal
Author
Source
Veterinarski Arhiv. July-August 2021, Vol. 91 Issue 4, p411, 15 p.
Subject
Language
English
ISSN
0372-5480
Abstract
Introduction Rheumatoid arthritis (RA) is a chronic systemic autoimmune disease, characterized by inflammation of joints, destruction of articular tissues, and deformation of fingers and palms. This disease requires sustained treatment, [...]
The present study evaluated the therapeutic benefits of complex herbal preparation named CBMDS, consisting of turmeric (Curcuma longa), Boswellia (Boswellia serrata), Methylsulphonylmethane, Devil's Claw (Harpagophytum procumbens) and Silymarin, using it in combination with methotrexate, in order to suppress adjuvant arthritis in rats, and to attenuate methotrexate-induced liver damage. Adjuvant arthritis was induced in 28 rats by a single subplantar injection of complete Freund's adjuvant (0.1 mL) into the left hind paw. The animals were divided into four groups (with seven animals in each). Group I received CBMDS, Group II - CBMDS in combination with methotrexate, and Group III just methotrexate. The treatment lasted from day 0 to day 17 (CBMDS was given daily except weekends in a dose of 160 mg/kg, methotrexate - 2 mg/kg once a week). Group IV was the control group. Clinical (body weight, hind paw volume, erythrocyte sedimentation rate, leukocyte count), biochemical (serum pro-/antioxidant activity markers), immunological (serum interleukin levels) and histological changes in joint and liver tissues were evaluated. CBMDS significantly alleviated arthritis and reduced hepatic damage, which was more evident in the methotrexate group. The combined treatment also markedly reduced arthritic symptoms and levels of malondialdehyde. Antioxidant activity was significantly higher in treated Groups I and II. CBMDS and its combination with methotrexate promoted anti-arthritic action, reduced histological changes in the joint tissues, and minimized methotrexate-induced liver toxicity. Key words: adjuvant arthritis; complex of herbal extracts; methotrexate; antioxidant activity U ovom je radu procijenjena terapijska korist mjeavine biljnih preparata pod imenom CBMDS, koja sadrava kurkumu (Curcuma longa), tamjanovo drvo (Boswellia serrata), metilsulfonilmetan, vraju kandu (Harpagophytum procumbens) i silimarin. Biljni preparati su upotrijebljeni u kombinaciji s metotrekstom s ciljem supresije adjuvantnog artritisa u takora i smanjenja otecenja jetre uzrokovanog metotreksatom. Adjuvantni artritis izazvan je u 28 takora pojedinacnom subplantarnom injekcijom mineralne uljne emulzije Freund's Complete Adjuvant (0,1 mL) u stranju lijevu apu. ivotinje su podijeljene u cetiri skupine (u svakoj po sedam jedinki). Skupina I primila je CBMDS, skupina II - CBMDS u kombinaciji s metotreksatom, a skupina III - metotreksat. Pokus je trajao od nultog do 17. dana (CBMDS je davan svaki dan, osim vikendom, u dozi od 160 mg/kg, metotreksat u dozi od 2 mg/kg jedanput tjedno). Skupina IV bila je kontrolna skupina. Procijenjene su klinicke (tjelesna masa, opseg stranje ape, sedimetacija eritrocita, broj leukocita), biokemijske (marker serumske aktivnosti prooksidansa i antioksidansa), imunoloke (razine serumskog interleukina) i histoloke promjene u zglobovima i jetri. CBMDS je znakovito ublaio artritis i smanjio otecenje jetre, to je bilo ocitije u skupini s metotreksatom. Kombinirana terapija takoder je znatno smanjila simptome artritisa i razine malondialdehida. Antioksidacijska aktivnost bila je znakovito veca u skupinama I i II. CBMDS u kombinaciji s metotreksatom imao je antiartriticno djelovanje, smanjio je histoloke promjene u zglobovima i minimizirao toksicnost metotreksata za jetru. Kljucne rijeci: adjuvantni artritis; kompleks biljnih ekstrakata; metotreksat; antioksidacijska aktivnost
The present study evaluated the therapeutic benefits of complex herbal preparation named CBMDS, consisting of turmeric (Curcuma longa), Boswellia (Boswellia serrata), Methylsulphonylmethane, Devil's Claw (Harpagophytum procumbens) and Silymarin, using it in combination with methotrexate, in order to suppress adjuvant arthritis in rats, and to attenuate methotrexate-induced liver damage. Adjuvant arthritis was induced in 28 rats by a single subplantar injection of complete Freund's adjuvant (0.1 mL) into the left hind paw. The animals were divided into four groups (with seven animals in each). Group I received CBMDS, Group II - CBMDS in combination with methotrexate, and Group III just methotrexate. The treatment lasted from day 0 to day 17 (CBMDS was given daily except weekends in a dose of 160 mg/kg, methotrexate - 2 mg/kg once a week). Group IV was the control group. Clinical (body weight, hind paw volume, erythrocyte sedimentation rate, leukocyte count), biochemical (serum pro-/antioxidant activity markers), immunological (serum interleukin levels) and histological changes in joint and liver tissues were evaluated. CBMDS significantly alleviated arthritis and reduced hepatic damage, which was more evident in the methotrexate group. The combined treatment also markedly reduced arthritic symptoms and levels of malondialdehyde. Antioxidant activity was significantly higher in treated Groups I and II. CBMDS and its combination with methotrexate promoted anti-arthritic action, reduced histological changes in the joint tissues, and minimized methotrexate-induced liver toxicity. Key words: adjuvant arthritis; complex of herbal extracts; methotrexate; antioxidant activity U ovom je radu procijenjena terapijska korist mjeavine biljnih preparata pod imenom CBMDS, koja sadrava kurkumu (Curcuma longa), tamjanovo drvo (Boswellia serrata), metilsulfonilmetan, vraju kandu (Harpagophytum procumbens) i silimarin. Biljni preparati su upotrijebljeni u kombinaciji s metotrekstom s ciljem supresije adjuvantnog artritisa u takora i smanjenja otecenja jetre uzrokovanog metotreksatom. Adjuvantni artritis izazvan je u 28 takora pojedinacnom subplantarnom injekcijom mineralne uljne emulzije Freund's Complete Adjuvant (0,1 mL) u stranju lijevu apu. ivotinje su podijeljene u cetiri skupine (u svakoj po sedam jedinki). Skupina I primila je CBMDS, skupina II - CBMDS u kombinaciji s metotreksatom, a skupina III - metotreksat. Pokus je trajao od nultog do 17. dana (CBMDS je davan svaki dan, osim vikendom, u dozi od 160 mg/kg, metotreksat u dozi od 2 mg/kg jedanput tjedno). Skupina IV bila je kontrolna skupina. Procijenjene su klinicke (tjelesna masa, opseg stranje ape, sedimetacija eritrocita, broj leukocita), biokemijske (marker serumske aktivnosti prooksidansa i antioksidansa), imunoloke (razine serumskog interleukina) i histoloke promjene u zglobovima i jetri. CBMDS je znakovito ublaio artritis i smanjio otecenje jetre, to je bilo ocitije u skupini s metotreksatom. Kombinirana terapija takoder je znatno smanjila simptome artritisa i razine malondialdehida. Antioksidacijska aktivnost bila je znakovito veca u skupinama I i II. CBMDS u kombinaciji s metotreksatom imao je antiartriticno djelovanje, smanjio je histoloke promjene u zglobovima i minimizirao toksicnost metotreksata za jetru. Kljucne rijeci: adjuvantni artritis; kompleks biljnih ekstrakata; metotreksat; antioksidacijska aktivnost