부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.gene.2005.06.046 Byline: Guohai Wu (a), Albert J. Robertson (b), Ping Zheng (a), Xunjia Liu (b), Lawrence V. Gusta (b) Keywords: Bromegrass; Peroxisome channel protein; ABA; Drought and cold stresses; Embryogenesis Abstract: A cDNA (BG-15) was isolated through differential screening of a cDNA library made from an ABA-treated bromegrass (Bromus inermis Leyss) suspension cell culture. The 819 bp pair cDNA encoded a 174 amino acid polypeptide with a calculated molecular mass of 18.08 kD and isolectric point of 7.50. The deduced amino acid sequences for the cDNA were 29.5% and 32.6% homologous to the known amino acid-selective channel proteins of the chloroplastic outer membrane in pea and barley, but were highly homologous (55.6% to 83.2%) to the putative membrane channel proteins from rice and Arabidopsis. Immunogold localization demonstrated that the channel protein encoded by this cDNA was present on the peroxisome membrane. High stringency southern analysis revealed that 1 to 2 copies of the peroxisomal channel protein (PCP) genes were present in the bromegrass genome. Northern and Western blots revealed that the PCP gene was responsive to both cold and drought stresses, and was rapidly induced by ABA (75 [mu]M). The transcript of the PCP gene also accumulated during late embryogenesis, but declined rapidly during germination. Data taken together, responsiveness of the PCP to cold and drought stresses, and accumulation during late embryogenesis suggest this novel peroxisomal channel protein is associated with sugar and fatty acid metabolism through fatty acid import or succinate export from peroxisome during desiccation tolerance and energy metabolism. Author Affiliation: (a) Bioriginal Food and Science Corporation, 110 Gymnasium Place, Saskatoon, Saskatchewan, Canada S7N 0W9 (b) Crop Development Center, University of Saskatchewan, 51 Campus Drive, Saskatoon, Canada SK, S7N 5A8 Article History: Received 25 April 2005; Accepted 16 June 2005 Article Note: (footnote) [star] The nucleotide sequence reported is in Genbank, NCBI Nucleotide Databases under the accession number AY057933.