학술논문
Ex vivo expansion of human umbilical cord blood hematopoietic progenitor cells in a novel three-dimensional culture system
Document Type
Academic Journal
Author
Source
Biotechnology Letters. Sept, 2002, Vol. 24 Issue 17, p1421, 6 p.
Subject
Language
English
ISSN
0141-5492
Abstract
A novel three-dimensional culture system for the ex vivo expansion of human umbilical cord blood (CB) hematopietic progenitor cells (HPCs) was developed by growing CB mononuclear cells on highly porous CultiSpher G microspheres coated with human bone marrow stromal cells in stirred flasks in the presence of supplemented cytokines. After 12 days, the number of total viable cells, colony-forming units in culture (CFU-C) and CD34.sup.+ cells present in the cultures reflected average increases of 7.7, 23.3 and 9.6-fold, respectively, and marked hematopoietic islands were formed on the surface of CultiSpher G.