부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.cellimm.2006.07.008 Byline: Zhaocai Yu, Wenchao Liu, Duhu Liu, Li Fan Keywords: Soluble interleukin-6 receptor; Interleukin-6; gp130; Myeloid; Erythroid; Hematopoiesis; Dendritic cell; Granulocytic cell; Monocytic cell; Hematopoietic stem cell Abstract: This study was designed to investigate the regulatory role of soluble interleukin-6 receptor (sIL-6R) and interleukin-6 (IL-6) fusion protein (Hyper-IL-6) in the differentiation of human myeloid and erythroid progenitors by a serum-free liquid suspension culture system, using the human cord blood-derived CD34.sup.+CD38.sup.- cells as a target. We found that Hyper-IL-6 promoted the generation of CD15.sup.+ granulocytic and CD14.sup.+ monocytic cells and suppressed that of CD14.sup.-CD1a.sup.+ dendritic cells from CD36.sup.-CD15.sup.-CD14.sup.-CD1a.sup.-IL-6R.sup.+ myeloid progenitors. Conversely, CD34.sup.+CD38.sup.- cell-derived early erythroid progenitors were negative for IL-6R expression. Hyper-IL-6 potentiated the generation of CD36.sup.+glycophorinA.sup.high mature erythroid cells from the IL-6R.sup.- early erythroid progenitors. Our results indicate that Hyper-IL-6 augments the generation of CD15.sup.+ granulocytic, CD14.sup.+ monocytic and CD36.sup.+glycophorinA.sup.high cell and suppresses that of CD14.sup.-CD1a.sup.+ dendritic cells. Author Affiliation: Department of Clinical Oncology, Xijing Hospital, Fourth Military Medical University, Xi'an 710032, China Article History: Received 27 May 2006; Accepted 18 July 2006