학술논문
An updated guide for the perplexed: cytometry in the high-dimensional era
Document Type
Academic Journal
Author
Source
Nature Immunology. October 2021, Vol. 22 Issue 10, p1190, 8 p.
Subject
Language
English
ISSN
1529-2908
Abstract
Author(s): Thomas Liechti [sup.1] , Lukas M. Weber [sup.2] , Thomas M. Ashhurst [sup.3] [sup.4] , Natalie Stanley [sup.5] , Martin Prlic [sup.6] , Sofie Van Gassen [sup.7] [sup.8] , [...]
High-dimensional cytometry experiments measuring 20-50 cellular markers have become routine in many laboratories. The increased complexity of these datasets requires added rigor during the experimental planning and the subsequent manual and computational data analysis to avoid artefacts and misinterpretation of results. Here we discuss pitfalls frequently encountered during high-dimensional cytometry data analysis and aim to provide a basic framework and recommendations for reporting and analyzing these datasets.
High-dimensional cytometry experiments measuring 20-50 cellular markers have become routine in many laboratories. The increased complexity of these datasets requires added rigor during the experimental planning and the subsequent manual and computational data analysis to avoid artefacts and misinterpretation of results. Here we discuss pitfalls frequently encountered during high-dimensional cytometry data analysis and aim to provide a basic framework and recommendations for reporting and analyzing these datasets.