학술논문
Genome evolution of dengue virus serotype 1 under selection by Wolbachia pipientis in Aedes aegypti mosquitoes
Document Type
Report
Author
Kien, Duong Thi Hue; Edenborough, Kathryn; Goncalves, Daniela da Silva; Vi, Tran Thuy; Casagrande, Etiene; Duyen, Huynh Thi Le; Long, Vo Thi; Dui, Le Thi; Nhu, Vu Thi Tuyet; Giang, Nguyen Thi; Trang, Huynh Thi Xuan; Lee, Elvina; Donovan-Banfield, I'ah; Van, Huynh Thi Thuy; Nguyet, Nguyen Minh; Phong, Nguyen Thanh; Chau, Nguyen Van Vinh; Wills, Bridget; Yacoub, Sophie; Flores, Heather; Simmons, Cameron
Source
Virus Evolution. January, 2023, Vol. 9 Issue 1, p1, 10 p.
Subject
Language
English
Abstract
The introgression of antiviral strains of Wolbachia into Aedes aegypti mosquito populations is a public health intervention for the control of dengue. Plausibly, dengue virus (DENV) could evolve to bypass the antiviral effects of Wolbachia and undermine this approach. Here, we established a serial-passage system to investigate the evolution of DENV in Ae. aegypti mosquitoes infected with the iiMel strain of Wolbachia. Using this system, we report on virus genetic outcomes after twenty passages of serotype 1 of DENV (DENV-1). An amino acid substitution, E203K, in the DENV-1 envelope protein was more frequently detected in the consensus sequence of virus populations passaged in wMel-infected Ae. aegypti than wild-type counterparts. Positive selection at residue 203 was reproducible; it occurred in passaged virus populations from independent DENV-1-infected patients and also in a second, independent experimental system. In wild-type mosquitoes and human cells, the 203K variant was rapidly replaced by the progenitor sequence. These findings provide proof of concept that wMel-associated selection of virus populations can occur in experimental conditions. Field-based studies are needed to explore whether wMel imparts selective pressure on DENV evolution in locations where wMel is established. Key words: dengue virus; wMel Wolbachia; virus evolution; Aedes aegypti mosquitoes; biocontrol.
Introduction Dengue is a public health burden in many tropical countries and is responsible for significant morbidity and health-care expenditure (Martelli et al. 2015; Stanaway et al. 2016; Wilastonegoro et [...]
Introduction Dengue is a public health burden in many tropical countries and is responsible for significant morbidity and health-care expenditure (Martelli et al. 2015; Stanaway et al. 2016; Wilastonegoro et [...]