학술논문
Community recommendations on cryoEM data archiving and validation
Document Type
Academic Journal
Author
Kleywegt, Gerard J.; Adams, Paul D.; Butcher, Sarah J.; Lawson, Catherine L.; Rohou, Alexis; Rosenthal, Peter B.; Subramaniam, Sriram; Topf, Maya; Abbott, Sanja; Baldwin, Philip R.; Berrisford, John M.; Bricogne, Gerard; Choudhary, Preeti; Croll, Tristan I.; Danev, Radostin; Ganesan, Sai J.; Grant, Timothy; Gutmanas, Aleksandras; Henderson, Richard; Heymann, J. Bernard; Huiskonen, Juha T.; Lstrate, Andrei; Kato, Takayuki; Lander, Gabriel C.; Lok, Shee-Mei; Ludtke, Steven J.; Murshudov, Garib N.; Pye, Ryan; Pintilie, Grigore D.; Richardson, Jane S.; Sachse, Carsten; Salih, Osman; Scheres, Sjors H.W.; Schroeder, Gunnar F.; Sorzano, Carlos Oscar S.; Stagg, Scott M.; Wang, Zhe; Warshamanage, Rangana; Westbrook, John D.; Winn, Martyn D.; Young, Jasmine Y.; Burley, Stephen K.; Hoch, Jeffrey C.; Kurisu, Genji; Morris, Kyle; Patwardhan, Ardan; Velankar, Sameer
Source
International Union of Crystallography Journal. March, 2024, Vol. 11 Issue 2, p140, 12 p.
Subject
Language
English
ISSN
2052-2525
Abstract
In January 2020, a workshop was held at EMBL-EBI (Hinxton, UK) to discuss data requirements for the deposition and validation of cryoEM structures, with a focus on single-particle analysis. The meeting was attended by 47 experts in data processing, model building and refinement, validation, and archiving of such structures. This report describes the workshop's motivation and history, the topics discussed, and the resulting consensus recommendations. Some challenges for future methods-development efforts in this area are also highlighted, as is the implementation to date of some of the recommendations. Keywords: cryogenic-specimen electron microscopy; data archiving; validation; quality control; Electron Microscopy Data Bank; Protein Data Bank; single-particle cryoEM; databases; bioinformatics; structure determination.
1. Introduction and background Structural biology, the study of the 3D structures of biological entities on scales from biomolecules to cells, has had an enormous impact on our understanding of [...]
1. Introduction and background Structural biology, the study of the 3D structures of biological entities on scales from biomolecules to cells, has had an enormous impact on our understanding of [...]