학술논문
The effect of leptin receptor deficiency and fasting on cannabinoid receptor 1 mRNA expression in the rat hypothalamus, brainstem and nodose ganglion
Document Type
Report
Source
Neuroscience Letters. Oct 2, 2009, Vol. 463 Issue 2, p125, 5 p.
Subject
Language
English
ISSN
0304-3940
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.neulet.2009.07.011 Byline: Jacob Jelsing (a)(b), Philip Just Larsen (a)(c), Niels Vrang (a)(b) Keywords: Fasting; Cannabinoid receptor 1; Zucker rat; Leptin; Hypothalamus; Brainstem; Nodose ganglion Abstract: Despite ample evidence for the involvement of the endocannabinoid system in the control of appetite, food intake and energy balance, relatively little is known about the regulation of cannabinoid receptor 1 (CB.sub.1R) expression in respect to leptin signalling and fasting. In the present study, we examined CB.sub.1R mRNA levels in lean (Fa/?) and obese (fa/fa) male Zucker rats under basal and food-restricted conditions. Using stereological sampling principles coupled with semi-quantitative radioactive in situ hybridization we provide semi-quantitative estimates of CB.sub.1R mRNA expression in key appetite regulatory hypothalamic and brainstem areas, as well as in the nodose ganglia. Whereas no effect of fasting were determined on CB.sub.1R mRNA levels in the paraventricular (PVN) and ventromedial hypothalamic (VMH) nucleus, in the brainstem dorsal vagal complex or nodose ganglion of lean Zucker rats, CB.sub.1R mRNA levels were consistently elevated in obese Zucker rats pointing to a direct influence of disrupted leptin signalling on CB.sub.1R mRNA regulation. Author Affiliation: (a) Rheoscience A/S, RA[cedilla]dovre, Denmark (b) Gubra, Copenhagen, Denmark (c) Eli Lilly, Indianapolis, USA Article History: Received 22 April 2009; Revised 2 July 2009; Accepted 4 July 2009