학술논문
Drug resistance phenotypes and genotypes in Mexico in representative gram-negative species: Results from the infivar network
Document Type
Report
Author
Garza-González, Elvira; Bocanegra-Ibarias, Paola; Bobadilla-del-Valle, Miriam; Ponce-de-León-Garduño, Luis Alfredo; Esteban-Kenel, Verónica; Silva-Sánchez, Jesus; Garza-Ramos, Ulises; Barrios-Camacho, Humberto; López-Jácome, Luis Esaú; Colin-Castro, Claudia A.; Franco-Cendejas, Rafael; Flores-Treviño, Samantha; Morfín-Otero, Rayo; Rojas-Larios, Fabian; Mena-Ramírez, Juan Pablo; Fong-Camargo, María Guadalupe; Morales-De-la-Peña, Cecilia Teresita; García-Mendoza, Lourdes; Choy-Chang, Elena Victoria; Aviles-Benitez, Laura Karina; Feliciano-Guzmán, José Manuel; López-Gutiérrez, Eduardo; Gil-Veloz, Mariana; Barajas-Magallón, Juan Manuel; Aguirre-Burciaga, Efren; López-Moreno, Laura Isabel; Martínez-Villarreal, Rebeca Thelma; Canizales-Oviedo, Jorge Luis; Cetina-Umaña, Carlos Miguel; Romero-Romero, Daniel; Bello-Pazos, Fidencio David; Barlandas-Rendón, Nicolás Rogelio Eric; Maldonado-Anicacio, Joyarib Yanelli; Bolado-Martínez, Enrique; Galindo-Méndez, Mario; Perez-Vicelis, Talia; Alavez-Ramírez, Norma; Méndez-Sotelo, Braulio J.; Cabriales-Zavala, Juan Francisco; Nava-Pacheco, Yirla Citlali; Moreno-Méndez, Martha Irene; García-Romo, Ricardo; Silva-Gamiño, Aldo Rafael; Avalos-Aguilera, Ana María; Santiago-Calderón, María Asunción; López-García, Maribel; Velázquez-Acosta, María del Consuelo; Cobos-Canul, Dulce Isabel; Vázquez-Larios, María del Rosario; Ortiz-Porcayo, Ana Elizabeth; Guerrero-Núñez, Arely Elizabeth; Valero-Guzmán, Jazmín; Rosales-García, Alina Aracely; Ostos-Cantú, Heidy Leticia; Camacho-Ortiz, Adrián
Source
PLoS ONE. March 17, 2021, Vol. 16 Issue 3, e0248614
Subject
Language
English
ISSN
1932-6203
Abstract
Aim This report presents phenotypic and genetic data on the prevalence and characteristics of extended-spectrum [beta]-lactamases (ESBLs) and representative carbapenemases-producing Gram-negative species in Mexico. Material and methods A total of 52 centers participated, 43 hospital-based laboratories and 9 external laboratories. The distribution of antimicrobial resistance data for Escherichia coli, Klebsiella pneumoniae, Enterobacter cloacae complex, Acinetobacter baumannii complex, and Pseudomonas aeruginosa in selected clinical specimens from January 1 to March 31, 2020 was analyzed using the WHONET 5.6 platform. The following clinical isolates recovered from selected specimens were included: carbapenem-resistant Enterobacteriaceae, ESBL or carbapenem-resistant E. coli, and K. pneumoniae, carbapenem-resistant A. baumannii complex, and P. aeruginosa. Strains were genotyped to detect ESBL and/or carbapenemase-encoding genes. Results Among blood isolates, A. baumannii complex showed more than 68% resistance for all antibiotics tested, and among Enterobacteria, E. cloacae complex showed higher resistance to carbapenems. A. baumannii complex showed a higher resistance pattern for respiratory specimens, with only amikacin having a resistance lower than 70%. Among K. pneumoniae isolates, bla.sub.TEM, bla.sub.SHV, and bla.sub.CTX were detected in 68.79%, 72.3%, and 91.9% of isolates, respectively. Among E. coli isolates, bla.sub.TEM, bla.sub.SHV, and bla.sub.CTX were detected in 20.8%, 4.53%, and 85.7% isolates, respectively. For both species, the most frequent genotype was bla.sub.CTX-M-15 . Among Enterobacteriaceae, the most frequently detected carbapenemase-encoding gene was bla.sub.NDM-1 (81.5%), followed by bla.sub.OXA-232 (14.8%) and bla.sub.oxa-181 (7.4%), in A. baumannii was bla.sub.OXA-24 (76%) and in P. aeruginosa, was bla.sub.IMP (25.3%), followed by bla.sub.GES and bla.sub.VIM (13.1% each). Conclusion Our study reports that NDM-1 is the most frequent carbapenemase-encoding gene in Mexico in Enterobacteriaceae with the circulation of the oxacillinase genes 181 and 232. KPC, in contrast to other countries in Latin America and the USA, is a rare occurrence. Additionally, a high circulation of ESBL bla.sub.CTX-M-15 exists in both E. coli and K. pneumoniae.
Author(s): Elvira Garza-González 1, Paola Bocanegra-Ibarias 1, Miriam Bobadilla-del-Valle 2, Luis Alfredo Ponce-de-León-Garduño 2, Verónica Esteban-Kenel 2, Jesus Silva-Sánchez 3, Ulises Garza-Ramos 3, Humberto Barrios-Camacho 3, Luis Esaú López-Jácome 4, [...]
Author(s): Elvira Garza-González 1, Paola Bocanegra-Ibarias 1, Miriam Bobadilla-del-Valle 2, Luis Alfredo Ponce-de-León-Garduño 2, Verónica Esteban-Kenel 2, Jesus Silva-Sánchez 3, Ulises Garza-Ramos 3, Humberto Barrios-Camacho 3, Luis Esaú López-Jácome 4, [...]