학술논문
Transforming growth factor-[beta]2 enhances differentiation of cardiac myocytes from embryonic stem cells
Document Type
Academic Journal
Author
Source
Biochemical and Biophysical Research Communications. June 24, 2005, Vol. 332 Issue 1, p135, 7 p.
Subject
Language
English
ISSN
0006-291X
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2005.04.098 Byline: Dinender Kumar, Baiming Sun Abstract: Stem cell therapy holds great promise for the treatment of injured myocardium, but is challenged by a limited supply of appropriate cells. Three different isoforms of transforming growth factor-[beta] (TGF-[beta]) -[beta]1, -[beta]2, and -[beta]3 exhibit distinct regulatory effects on cell growth, differentiation, and migration during embryonic development. We compared the effects of these three different isoforms on cardiomyocyte differentiation from embryonic stem (ES) cells. In contrast to TGF-[beta]1, or -[beta]3, treatment of mouse ES cells with TGF-[beta]2 isoform significantly increased embryoid body (EB) proliferation as well as the extent of the EB outgrowth that beat rhythmically. At 17 days, 49% of the EBs treated with TGF-[beta]2 exhibited spontaneous beating compared with 15% in controls. Cardiac myocyte specific protein markers sarcomeric myosin and [alpha]-actin were demonstrated in beating EBs and cells isolated from EBs. In conclusion, TGF-[beta]2 but not TGF-[beta]1, or -[beta]3 promotes cardiac myocyte differentiation from ES cells. Author Affiliation: Department of Medicine, University of Vermont, Burlington, VT 05446, USA