학술논문
1, 25-(OH).sub.2D.sub.3 protects against ER stress and miRNA dysregulation in Mus musculus neurons
Research Article
Research Article
Document Type
Academic Journal
Author
Source
Genes & Genomics. December 2022, Vol. 44 Issue 12, p1565, 12 p.
Subject
Language
English
ISSN
1976-9571
Abstract
Author(s): Parvathy Jayachandran [sup.1], Linda Koshy [sup.1], Perumana R. Sudhakaran [sup.1], Govindapillai Mohanadasan Nair [sup.1], Appukuttan Nair Gangaprasad [sup.1] [sup.2], Ananthakrishnan Jayakumaran Nair [sup.1] Author Affiliations: (1) grid.413002.4, 0000 0001 [...]
Background The pathophysiology of neurodegenerative diseases (NDDs) is closely associated with cellular oxidative stress which can result in the accumulation of toxic proteins in the endoplasmic reticulum (ER) leading to ER stress and subsequent unfolded protein response (UPR) signaling, a mechanism that aggravate these disorders. Vitamin D has been suggested to have important neuroprotective role and its administration has been shown to reduce neuronal injury, neurotoxicity and oxidative stress in various animal systems. Objective The current study was undertaken to examine the effect of vitamin D.sub.3 on UPR in ER stress induced Mus musculus neuronal cells. Methods Mus musculus cortical and hippocampal primary neuronal cultures were pretreated with 1,25-dihydroxyvitamin D.sub.3 (1, 25-(OH).sub.2D.sub.3), the active form of vitamin D, followed by ER stress induction with a chemical ER stress inducer thapsigargin and with an advanced glycated protein, AGE-BSA. The UPR genes and related microRNAs (miRNA) expressions were analyzed mainly using real-time PCR. Results The experiment resulted in the suppression of ER stress marker BiP and UPR pathway genes such as Perk, Ire1[alpha], Chop and Puma which mediate cellular apoptosis indicating the protective effect of 1, 25-(OH).sub.2D.sub.3 against neuronal ER stress. Further studies into the molecular aspects showed that ER stress mediated down-regulated expression of microRNAs (miRNAs) such as mmu-miR-24, 27b, 124, 224, 290, 351 and 488 which are known to regulate the UPR pathway genes were also reduced with vitamin pretreatment, of which the miRNAs miR-24 and 27b which shares the same cluster are potentially involved in various human diseases. Conclusion This study emphasizes the therapeutic role of vitamin D in reducing neuronal ER stress and the need for maintaining sufficient amount of this vitamin in our diet.
Background The pathophysiology of neurodegenerative diseases (NDDs) is closely associated with cellular oxidative stress which can result in the accumulation of toxic proteins in the endoplasmic reticulum (ER) leading to ER stress and subsequent unfolded protein response (UPR) signaling, a mechanism that aggravate these disorders. Vitamin D has been suggested to have important neuroprotective role and its administration has been shown to reduce neuronal injury, neurotoxicity and oxidative stress in various animal systems. Objective The current study was undertaken to examine the effect of vitamin D.sub.3 on UPR in ER stress induced Mus musculus neuronal cells. Methods Mus musculus cortical and hippocampal primary neuronal cultures were pretreated with 1,25-dihydroxyvitamin D.sub.3 (1, 25-(OH).sub.2D.sub.3), the active form of vitamin D, followed by ER stress induction with a chemical ER stress inducer thapsigargin and with an advanced glycated protein, AGE-BSA. The UPR genes and related microRNAs (miRNA) expressions were analyzed mainly using real-time PCR. Results The experiment resulted in the suppression of ER stress marker BiP and UPR pathway genes such as Perk, Ire1[alpha], Chop and Puma which mediate cellular apoptosis indicating the protective effect of 1, 25-(OH).sub.2D.sub.3 against neuronal ER stress. Further studies into the molecular aspects showed that ER stress mediated down-regulated expression of microRNAs (miRNAs) such as mmu-miR-24, 27b, 124, 224, 290, 351 and 488 which are known to regulate the UPR pathway genes were also reduced with vitamin pretreatment, of which the miRNAs miR-24 and 27b which shares the same cluster are potentially involved in various human diseases. Conclusion This study emphasizes the therapeutic role of vitamin D in reducing neuronal ER stress and the need for maintaining sufficient amount of this vitamin in our diet.