부산대학교 도서관

Introduction Ticks, are worldwide non species specific ectoparasites of vertebrates. As blood-feeding arthropods, ticks threaten human health and due to their negative effects on the livestock cause economic losses. They [...]
Ticks are parasites and vectors, whose diet is blood and hosts are vertebrates. Therefore, they are a risk factor for both, public health and farm animals. Ticks have a cosmopolitan distribution, and their prevalence and incidence of diseases caused by them are increasing with the effect of various environmental factors such as global warming. The use of endosymbiotic bacteria (EB) to control pests is a promising environment-friendly approach as an alternative to chemical methods. Therefore, it is necessary to elucidate the ticks symbionts to develop potentially alternative strategies for managing their populations. Aim of this study, was to search for EB in ticks obtained from livestock in Kirsehir, Central Anatolia. EB were identified by morphological and molecular methods. Investigation was conducted on most studied EB in Haemaphysalis spp. and Hyalomma spp. using Arsenophonus, Cardinium, Hamiltonella, Rickettsia, Spiroplasma and Wolbachia specific primers. As a result of the survey, Rickettsia was detected in all locations and samples, while Spiroplasma was detected only one sampling locality. It was that Rickettsia is not species specific and has a wide wide distribution incidence. Spiroplasma was found only in Ha. sulcata. In contrary Arsenophonus, Cardinium, Hamiltonella and Wolbachia were not found in the sampled ticks. On the other hand, although the reason could not be explained, PCR products thought to be the result of non-specific binding with Arsenophonus primers and sequence data similar to Coxiella-like endosymbiont were obtained. This study does not explain the tick-symbiosis relationship, but the findings are considered important for future studies of tick biology and/or tick-borne diseases. Key words: endosymbiotic bacteria; Rickettsia; Spiroplasma; tick Krpelji su paraziti i vektori koji se hrane krvlju, a domacini su im kraljeznjaci. Zbog toga su rizican cimbenik i za javno zdravlje i za zivotinje u farmskom uzgoju. Krpelji se nalaze sirom svijeta, a prevalencija i incidencija bolesti koje uzrokuju u porastu su zbog utjecaja razlicitih cimbenika okolisa, poput globalnog zatopljenja. Upotreba endosimbiotskih bakterija (EB) u svrhu kontrole stetocina obecavajuca je ekoloski prihvatljiva alternativa kemijskim metodama. Kako bi se razvile potencijalne alternativne strategije za upravljanje populacijama krpelja, potrebno je razjasniti njihove simbionte. Cilj je ovog istrazivanja bio izdvojiti EB iz krpelja pronadenih u stoke u Kirsehir pokrajini Srednje Anadolije. Endosimbiotske bakterije identificirane su morfoloskim i molekularnim metodama. Istrazivanje je provedeno na najcesce promatranim endosimbiotskim bakterijama u vrstama Haemaphysalis spp. i Hyalomma spp. upotrebom primera specificnih za Arsenophonus, Cardinium, hamiltonele, rikecije, spiroplazme i volbahije. Kao rezultat istrazivanja rikecija je pronadena na svim mjestima i u svim uzorcima, a spiroplazma na samo jednom mjestu uzorkovanja. Pokazalo se da rikecija nije specificna za vrste i da je siroko rasprostranjena. Spiroplazma je pronadena samo u vrsti Ha. sulcata. Nasuprot tome, Arsenophonus, Cardinium, hamiltonela i volbahija nisu pronadeni u uzorcima krpelja. S druge strane, premda razlog nije otkriven, dobiveni su produkti PCR-a za koje se smatralo da su rezultat nespecificnog vezanja s primerima Arsenophonus i sekvencom koja se podudara s Coxiella-slicnom endosimbiontom. Ovo istrazivanje ne objasnjava odnos izmedu krpelja i simbionata, no rezultati se smatraju vaznima za buduca istrazivanja biologije krpelja i bolesti koje oni prenose. Kljucne rijeci: endosimbiotske bakterije; rikecija; spiroplazma; krpelj