부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1601-0825.2008.01451.x Byline: MK Frederic (1), T Yamaai (2), N Mizukawa (1), Y Kaneda (1), N Katase (3), M Gunduz (3), H Nagatsuka (3), T Sugahara (1) Keywords: HBD-1; HBD-2; HBD-3; mucocele; immunohistochemistry Abstract: Objectives and design: The expressions of human beta defensin-1 (HBD-1), -2 (HBD-2) and -3 (HBD-3) in non-inflamed pseudocysts such as mucoceles were investigated immunohistochemically in this study. Materials and methods: Mucocele specimens were obtained from 21 patients. The expression of HBDs was studied immunohistochemically by using antibodies directed against HBD-1, -2, and -3. Statistical analyses were carried out on serial sections stained with antibodies. Results: Cells expressing HBDs were found in mucoceles. The expression of HBD-2 was observed in floating cells in all the specimens, whereas HBD-1 and HBD-3-expressing cells were detected in 93% and 73% of the mucoceles, respectively. The HBD-2 signal was the most intense and the HBD-3 signal intensity was weaker than that of HBD-1. HBDs were expressed in neutrophils and in other floating cells. Interestingly, the signal intensity and the population of positive cells located close to the centers of cysts were higher than those located in the peripheral areas of cysts. Conclusion: The expression of HBDs was found even in non-inflamed pseudocysts such as mucoceles. These results suggest that an unknown mechanism not involved in biophylaxis for the expression of HBDs may exist. Author Affiliation: Departments of(1)Oral and Maxillofacial Reconstructive Surgery (2)Oral Function and Anatomy (3)Oral Pathology, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, Okayama, Japan Article History: Received 11 December 2007; revised 6 March 2008; accepted 25 March 2008 Article note: Dr N Mizukawa, Department of Oral and Maxillofacial, Reconstructive Surgery, Okayama University Graduate School of Medicine, Dentistry and Pharmaceutical Sciences, 2-5-1 Shikata-cho, Okayama 700-8525, Japan. Tel: +81 86 235 6697, Fax: +81 86 235 6699, E-mail: gmd17131@cc.okayama-u.ac.jp