학술논문
The mesoSPIM initiative: open-source light-sheet microscopes for imaging cleared tissue
Document Type
Report
Author
Source
Nature Methods. November 2019, p1105, 4 p.
Subject
Language
English
ISSN
1548-7091
Abstract
Author(s): Fabian F. Voigt [sup.1] [sup.2] , Daniel Kirschenbaum [sup.3] , Evgenia Platonova [sup.4] , St?phane Pag?s [sup.5] [sup.6] , Robert A. A. Campbell [sup.7] , Rahel Kastli [sup.1] [sup.2] [...]
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ((http://www.mesospim.org)). The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.
Light-sheet microscopy is an ideal technique for imaging large cleared samples; however, the community is still lacking instruments capable of producing volumetric images of centimeter-sized cleared samples with near-isotropic resolution within minutes. Here, we introduce the mesoscale selective plane-illumination microscopy initiative, an open-hardware project for building and operating a light-sheet microscope that addresses these challenges and is compatible with any type of cleared or expanded sample ((http://www.mesospim.org)). The mesoSPIM is an open hardware axially scanned light-sheet microscope for the rapid imaging of large cleared samples with isotropic resolution.