학술논문
Collapse of parallel folding channels in dihydrofolate reductase from Escherichia coli by site-directed mutagenesis
Document Type
Academic Journal
Source
Biochemistry. Dec 14, 1993, Vol. 32 Issue 49, p13566, 9 p.
Subject
Language
ISSN
0006-2960
Abstract
A four-intermediate set is transformed to a set of native conformers through four parallel channel during the folding of dihydrofolate reductase from Escherichia coli. The inhibitor, methotrexate, promotes refolding and two slow reactions occur through two parallel channels. This suggests that four parallel channels disintegrate to form only two parallel channel in the mutant.