부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.imlet.2006.12.004 Byline: Sylva Janovska, Ivona Pavkova, Martin Hubalek, Juraj LenAo, AleA Macela, JiAi Stulik Keywords: Francisella tularensis; Antigens; Vaccine Abstract: Francisella tularensis is a Gram-negative, facultative intracellular bacterium causing disease in many mammalian species. The low infectious dose of F. tularensis and the ease of air-borne transmission are the main features responsible for the classification of this bacterium as a potential biological weapon. The live attenuated strain of F. tularensis live vaccine strain (LVS) is currently only effective vaccine against tularemia, however, this type of vaccine has not been approved for human use. In the presented study, sub-immunoproteome analysis was performed to search for new immunogenic proteins of Francisella tularensis LVS grown under different conditions. By this approach 35 immunoreactive antigens were identified, 19 of them showed to be novel immunogens. In conclusion, sub-immunoproteome analysis resulted in successful identification of novel immunoreactive proteins. Author Affiliation: Institute of Molecular Pathology, Faculty of Military Health Sciences, University of Defence, Trebesska 1575, Hradec Kralove, Czech Republic Article History: Received 16 August 2006; Revised 15 December 2006; Accepted 17 December 2006