학술논문
Probing protein tertiary structure with amidination
Document Type
Author Abstract
Source
Analytical Chemistry. Nov 15, 2005, Vol. 77 Issue 22, p7274, 8 p.
Subject
Language
English
ISSN
0003-2700
Abstract
A chemical derivatization method, amidination, that has recently been effectively employed in peptide mass spectrometry experiments is used to covalently modify lysines in several standard proteins. Protein and peptide mass spectra identify sites at which the reaction does or does not occur. This is therefore a rapid approach to elucidate solvent-accessible regions of folded proteins.