부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1600-0714.2007.00602.x Byline: Bina Raju, Sivakami Rethnam Haug, Salah Osman Ibrahim, Karin Johanne Heyeraas Keywords: interstitial fluid pressure; lymph vessels; tongue cancer Abstract: Background: Interstitial fluid pressure (IFP) in most tumors is high, and this high pressure has been correlated with poor prognosis. Measurements of IFP in normal tongue and in tongue cancer are lacking. Recent research suggests the existence of a relationship between increased peritumoral lymph vessels (PTLV) and survival, and a correlation of increased lymphatic vessel density with an unfavorable prognosis has been reported. Materials and methods: In the present study, tongue squamous cell carcinoma (SCC) was induced by adding the carcinogen 4-nitroquinoline oxide in drinking water for 19 weeks. The IFP was measured by micropuncture and immunohistochemistry was used to visualize lymph vessels. Results: In normal tongue, IFP averaged 3.1 [+ or -] 0.3 mmHg. The IFP, both in the tumor (29.1 [+ or -] 2.9 mmHg) and 0.5 cm anterior to it (15.4 [+ or -] 2.1 mmHg) was consistently increased (P < 0.005) with values ranging from 10 to 40 mmHg. The highest IFP values were measured in rats with large tumors (P < 0.05) and low body weight (P < 0.001), suggesting that IFP increases with cancer progression. Lymphatic vessel area (%), as determined with the lymphatic specific marker LYVE-1 antibody, was significantly increased in the peritumoral area when compared to intratumoral and control mucosa (P < 0.05). There was a significant positive correlation between IFP, PTLV area, tumor size and invasiveness. Conclusions: Our data show that IFP is increased in tongue cancer. Corresponding changes in PTLV area, invasiveness, tumor area and IFP suggest that the increased pressure is caused by defective lymph drainage and solid stress generated by tumor cells growing in a low compliant environment. Article History: Accepted for publication June 17, 2007 Article note: Bina Raju, Department of Biomedicine, Section for Physiology, University of Bergen, 91 Jonas Lies Vei, N-5009, Bergen, Norway. Tel: 55586453, Fax: 55586360, E-mail: bina.raju@biomed.uib.no