학술논문
Diagnostic utility of total PSA, free PSA, percentage PSA, histology biopsy and expression pattern of MALAT-1 in prostate cancer patients in Southern Nigeria
ORIGINAL ARTICLE
ORIGINAL ARTICLE
Document Type
Academic Journal
Source
New Zealand Journal of Medical Laboratory Science. April 2020, Vol. 74 Issue 1, p3, 7 p.
Subject
Language
English
ISSN
1171-0195
Abstract
N Z J Med Lab Sci 2020; 74: 03-09 INTRODUCTION Prostate cancer is the second leading cause of cancer related deaths worldwide (1). It has been reported that prostate cancer [...]
Objective: This study examines the diagnostic utility of serum levels of total, free and percentage prostate specific antigen (PSA), histological biopsies and metastasis-associated lung adenocarcinoma transcript 1 gene (MALAT-1). Methods: Fifty-two patients were recruited for this study from the urology clinics of a tertiary and private based Hospital in Benin City. Serum samples obtained from participants were used to assay for total and free PSA by enzyme linked immunosorbent assay (ELISA). The expression pattern of MALAT-1 was detected using reverse transcriptase polymerase chain reaction (RT-PCR). Histological biopsy was done for 39 participants whose PSA levels were greater than 10ng/ml. A one-way ANOVA post hoc multiple comparisons and Student's t-test were used for comparison of groups while receivers operating characteristics (ROC) curve were used to determine their diagnostic utility. Results: There was a significant up regulation of MALAT-1 gene in all prostate cancer patients and some benign prostatic hyperplasia (BPH) patients compared to controls (P Conclusion: This study revealed that MALAT-1 gene is a better biomarker for prostate cancer risk, diagnosis and prognosis. Key words: Prostate cancer, PSA, MALAT-1 gene, reverse transcriptase polymerase chain reaction.
Objective: This study examines the diagnostic utility of serum levels of total, free and percentage prostate specific antigen (PSA), histological biopsies and metastasis-associated lung adenocarcinoma transcript 1 gene (MALAT-1). Methods: Fifty-two patients were recruited for this study from the urology clinics of a tertiary and private based Hospital in Benin City. Serum samples obtained from participants were used to assay for total and free PSA by enzyme linked immunosorbent assay (ELISA). The expression pattern of MALAT-1 was detected using reverse transcriptase polymerase chain reaction (RT-PCR). Histological biopsy was done for 39 participants whose PSA levels were greater than 10ng/ml. A one-way ANOVA post hoc multiple comparisons and Student's t-test were used for comparison of groups while receivers operating characteristics (ROC) curve were used to determine their diagnostic utility. Results: There was a significant up regulation of MALAT-1 gene in all prostate cancer patients and some benign prostatic hyperplasia (BPH) patients compared to controls (P Conclusion: This study revealed that MALAT-1 gene is a better biomarker for prostate cancer risk, diagnosis and prognosis. Key words: Prostate cancer, PSA, MALAT-1 gene, reverse transcriptase polymerase chain reaction.