학술논문
Polyethylene glycol‐based ultrasonic‐assisted enzymatic extraction, characterization, and antioxidant activity in vitro and in vivo of polysaccharides from Lonicerae japonica leaves
Document Type
Report
Author
Source
Food Science & Nutrition. September 13, 2019, Vol. 7 Issue 11, p3452, 11 p.
Subject
Language
English
ISSN
2048-7177
Abstract
INTRODUCTION Lonicera japonica can be deciduous and evergreen shrubs, or climbers with twining stems. It is widely distributed in eastern Asia countries such as Japan, Korea, and China, which is [...]
: In this paper, polyethylene glycol (PEG)‐based ultrasonic‐assisted enzymatic extraction (UAEE) was employed in polysaccharides extraction from Lonicerae japonica leaves (LJLP). The optimal extraction conditions (extraction time of 33 min, PEG concentration of 30%, and ultrasonic power of 191 W) were obtained by Box–Behnken design (BBD). Under this condition, the LJLP yield was 14.76%. Furthermore, LJLP was identified as a typical heteropolysaccharide considering the main constitutive monosaccharides include galactose (32.3%), glucose (20.9%), and ribose (15.2%). Moreover, LJLP exhibited high total reducing power and considerable scavenging activities on superoxide radicals, in a concentration‐dependent manner in vitro. In addition, antioxidant analysis in vivo revealed that for the LJLP‐treated mice, the superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), catalase (CAT) activities, and total antioxidant capacity (TAOC) were significantly increased, while the level of malondialdehyde (MDA) was decreased in both serum and liver.
: In this paper, polyethylene glycol (PEG)‐based ultrasonic‐assisted enzymatic extraction (UAEE) was employed in polysaccharides extraction from Lonicerae japonica leaves (LJLP). The optimal extraction conditions (extraction time of 33 min, PEG concentration of 30%, and ultrasonic power of 191 W) were obtained by Box–Behnken design (BBD). Under this condition, the LJLP yield was 14.76%. Furthermore, LJLP was identified as a typical heteropolysaccharide considering the main constitutive monosaccharides include galactose (32.3%), glucose (20.9%), and ribose (15.2%). Moreover, LJLP exhibited high total reducing power and considerable scavenging activities on superoxide radicals, in a concentration‐dependent manner in vitro. In addition, antioxidant analysis in vivo revealed that for the LJLP‐treated mice, the superoxide dismutase (SOD), glutathione peroxidase (GSH‐Px), catalase (CAT) activities, and total antioxidant capacity (TAOC) were significantly increased, while the level of malondialdehyde (MDA) was decreased in both serum and liver.