부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.neulet.2008.05.051 Byline: Yoo Jung Yang (a), Hak Ju Lee (b), Don Ha Choi (b), Hai Shan Huang (a), Sung Cil Lim (a), Myung Koo Lee (a) Keywords: Scoparone; Neurite outgrowth; Cyclic AMP; PC12 cells Abstract: The neurite outgrowth-promoting effects of scoparone isolated from the stem bark of Liriodendron tulipifera were investigated in PC12 cells. At a concentration of 200[mu]M, scoparone markedly induced neurite outgrowth from PC12 cells. Scoparone at 200[mu]M also enhanced the outgrowth of neurites from cells in the presence of nerve growth factor (NGF, 2ng/ml). The levels of intracellular cyclic AMP and concentration of Ca.sup.2+ were also increased by 200[mu]M scoparone. In addition, scoparone at 200[mu]M increased the activities of extracellular signal-regulated protein kinase (ERK), cyclic AMP-dependent protein kinase (PKA), protein kinase C (PKC) and Ca.sup.2+/calmodulin kinase II (CaMK II). However, scoparone-induced neurite outgrowth was blocked by a mitogen-activated protein kinase inhibitor (U0126), a PKA inhibitor (H89), a PKC inhibitor (GF109203X) and a CaMK II inhibitor (KN62). These kinase inhibitors also reduced the scoparone-induced neurite outgrowth associated with NGF. These results suggest that scoparone can induce neurite outgrowth by stimulating the upstream steps of ERK, PKA, PKC and CaMK II in PC12 cells. Author Affiliation: (a) College of Pharmacy and Research Center for Bioresource and Health, Chungbuk National University, Cheongju 361-763, Republic of Korea (b) Division of Wood Chemistry and Microbiology, Korea Forest Research Institute, Seoul 130-712, Republic of Korea Article History: Received 2 April 2008; Revised 8 May 2008; Accepted 8 May 2008