학술논문
qAC2, a novel QTL that interacts with Wx and controls the low amylose content in rice (Oryza sativa L.)
Original Paper
Original Paper
Document Type
Report
Author
Source
Theoretical and Applied Genetics. April 2015, Vol. 128 Issue 4, p563, 11 p.
Subject
Language
English
ISSN
0040-5752
Abstract
Author(s): Yoko Takemoto-Kuno[sup.1] , Hiroki Mitsueda[sup.2] , Keitaro Suzuki[sup.1] , Hideyuki Hirabayashi[sup.1] , Osamu Ideta[sup.3] , Noriaki Aoki[sup.4] , Takayuki Umemoto[sup.5] , Takuro Ishii[sup.1] , Ikuo Ando[sup.1] , Hiroshi Kato[sup.6] [...]
Key message This manuscript reports the fine mapping of a novel QTL,qAC2controlling the low amylose in rice. The action mechanism of theqAC2is also investigated by the analysis of genetic interactions toWx.sup.a, Wx.sup.b, du1, du2anddu3. Amylose content of the rice (Oryza sativa L.) endosperm greatly affects starch properties and eating quality of cooked rice. Seeds of japonica rice cultivar Kuiku162 have low amylose content (AC) and good eating quality. Our analysis revealed a novel QTL, designated as qAC2 that contributed to the low AC of Kuiku162. qAC2 was fine mapped within a 74.9-kb region between two insertion and deletion markers, KID3001 and KID5101, on the long arm of chromosome 2. Seven genes are predicted in this region, but none of them is known to be related to the regulation of AC. The AC of a near-isogenic line (NIL110) carrying qAC2.sup.Kuiku, the Kuiku162 allele of qAC2, in the genetic background of japonica cultivar Itadaki was lower by 1.1 % points than that of Itadaki. The chain length distributions of amylopectin were similar in NIL110 and Itadaki; therefore, the low AC of NIL110 was caused by a decrease in the actual AC, but not by a difference in the amylopectin structure. The interaction analyses revealed that qAC2.sup.Kuiku has epistatic interaction with Wx.sup.a. The qAC2.sup.Kuiku has epistatic interactions with two loci, du1 and du2, on Wx.sup.b, whereas the genetic effect of qAC2.sup.Kuiku has additive to that of du3 on Wx.sup.b. Thus, similar to du1 and du2, qAC2 may have a function related to Wx.sup.b mRNA splicing. Electronic supplementary material The online version of this article (doi:10.1007/s00122-014-2432-6) contains supplementary material, which is available to authorized users.
Key message This manuscript reports the fine mapping of a novel QTL,qAC2controlling the low amylose in rice. The action mechanism of theqAC2is also investigated by the analysis of genetic interactions toWx.sup.a, Wx.sup.b, du1, du2anddu3. Amylose content of the rice (Oryza sativa L.) endosperm greatly affects starch properties and eating quality of cooked rice. Seeds of japonica rice cultivar Kuiku162 have low amylose content (AC) and good eating quality. Our analysis revealed a novel QTL, designated as qAC2 that contributed to the low AC of Kuiku162. qAC2 was fine mapped within a 74.9-kb region between two insertion and deletion markers, KID3001 and KID5101, on the long arm of chromosome 2. Seven genes are predicted in this region, but none of them is known to be related to the regulation of AC. The AC of a near-isogenic line (NIL110) carrying qAC2.sup.Kuiku, the Kuiku162 allele of qAC2, in the genetic background of japonica cultivar Itadaki was lower by 1.1 % points than that of Itadaki. The chain length distributions of amylopectin were similar in NIL110 and Itadaki; therefore, the low AC of NIL110 was caused by a decrease in the actual AC, but not by a difference in the amylopectin structure. The interaction analyses revealed that qAC2.sup.Kuiku has epistatic interaction with Wx.sup.a. The qAC2.sup.Kuiku has epistatic interactions with two loci, du1 and du2, on Wx.sup.b, whereas the genetic effect of qAC2.sup.Kuiku has additive to that of du3 on Wx.sup.b. Thus, similar to du1 and du2, qAC2 may have a function related to Wx.sup.b mRNA splicing. Electronic supplementary material The online version of this article (doi:10.1007/s00122-014-2432-6) contains supplementary material, which is available to authorized users.