부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1601-0825.2006.01358.x Byline: LA Rocha (1), PA Vargas (1), LFF Silva (2), JE Leon (1), AB Santos (2), PS Hiemstra (3), T Mauad (2) Keywords: secretory leukocyte proteinase inhibitor; submandibular gland; AIDS; immunohistochemistry; cytomegalovirus; autopsy Abstract: Objective: Secretory leukocyte proteinase inhibitor (SLPI) is an endogenous proteinase inhibitor present in mucosal secretions. It also displays antimicrobial activity including anti-human immunodeficiency virus activity. This protease inhibitor is also expressed in submandibular glands (SMG), but there are few data on its expression in AIDS patients with infectious conditions. Methods: We analyzed the expression of SLPI using immunohistochemistry in submandibular gland samples of 36 AIDS patients [10 with normal histology, 10 with chronic nonspecific sialadenitis, eight with mycobacteriosis, and eight with cytomegalovirus (CMV) infection] and 10 HIV-negative controls. The proteinase inhibitor was quantified using image analysis and expressed as % of positively stained area. Results: There was a higher expression of SLPI in AIDS patients with CMV infection (% of stained area, mean [+ or -] SD: 37.37 [+ or -] 14.45) when compared with all other groups (P = 0.009). There were no significant differences between control subjects (22.70 [+ or -] 9.42%) and AIDS patients without histologic alterations (18.10 [+ or -] 7.58%), with chronic nonspecific sialadenitis (17.13 [+ or -] 5.36%), or mycobacterial infection (21.09 [+ or -] 4.66%). Conclusion: Cytomegalovirus infection increases SLPI expression in the SMG of AIDS patients. Our results reveal new insights into the pathogenic association between HIV and CMV in AIDS patients. Author Affiliation: (1)Department of Oral Pathology, Faculty of Odontology of Piracicaba- University of Campinas, Piracicaba, SP, Brazil (2)Department of Pathology, Sao Paulo University Medical School, SP, Brazil (3)Department of Pulmonology, Leiden University Medical Center, Leiden, The Netherlands Article History: Received 21 July 2006; revised 18 October 2006; accepted 2 November 2006 Article note: T Mauad, Department of Pathology, Sao Paulo University Medical School, Av Dr Arnaldo, 455 1st floor, CEP: 01246-903, Sao Paulo, SP, Brazil. Tel: +55 11 30617173, Fax: +55 11 30642744, E-mail: tmauad@usp.br