학술논문
Polymerase chain reaction amplification of napthalene catabolic and 16S rRNA gene sequences from indigenous sediment bacteria
Document Type
Academic Journal
Source
Applied and Environmental Microbiology. March, 1993, Vol. 59 Issue 3, p687, 8 p.
Subject
Language
ISSN
0099-2240
Abstract
The utilization of polymerase chain reaction (PCR) to amplify bacterial genes from uninoculated surface and subsurface sediments is discussed. Bacterial strains for the experiment were drived from Pseudomonas putida and was subjected to a napthalene-contaminated field site. DNA extraction with gel electrophoresis was used to render the bacteria amplifiable by PCR. The utility of PCR for studies on function- and taxon specific DNA was established.