학술논문

Fusarium graminearum on plant cell wall: No fewer than 30 xylanase genes transcribed
Document Type
Report
Source
Biochemical and Biophysical Research Communications. July 7, 2006, Vol. 345 Issue 3, p959, 8 p.
Subject
Polymerase chain reaction
Enzymes
Monosaccharides
Sugars
Gene expression
Language
English
ISSN
0006-291X
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2006.04.171 Byline: Didier Hatsch (a), Vincent Phalip (b), Elizabet Petkovski (a), Jean-Marc Jeltsch (b) Keywords: Fungi; Gibberella; Cell wall degrading enzyme; Hemicellulase; Gene expression; Quantitative PCR Abstract: The transcription of a set of 32 putative xylanase genes from Fusarium graminearum was examined by quantitative PCR after growth on different carbon sources (hop cell wall, xylan, xylose, or carboxymethylcellulose). Growing on plant cell wall medium, this fungus displays a great diversity of expression of xylan-related genes, with 30 being induced. A second level of diversity exists because expression patterns can be very different for loci encoding enzymes with the same activity (the same EC number). The wealth of xylan-degrading enzymes and the differential expression confer on the fungus a great flexibility of reaction to variation in its environment. Author Affiliation: (a) Institut de Medecine Legale, Universite Louis Pasteur, 11 rue Humann, 67085 Strasbourg Cedex, France (b) Laboratoire de Phytopathologie, UMR 7175, Ecole Superieure de Biotechnologie de Strasbourg - Universite Louis Pasteur, Boulevard Sebastien Brandt, BP 10413, 67412 Illkirch-Graffenstaden Cedex, France Article History: Received 12 April 2006 Article Note: (footnote) [star] Abbreviations: CMC, carboxymethylcellulose; CWDE, cell wall degrading enzymes; ORF, open-reading frame.