부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.fgb.2007.12.002 Byline: RaphaA'l Carapito (a), Didier Hatsch (b), Sonja Vorwerk (c), Elizabet Petkovski (d), Jean-Marc Jeltsch (a), Vincent Phalip (a) Keywords: Gibberella zeae; Filamentous fungi; Humulus lupulus; Microarray; Suppressive subtractive hybridization; Subtracted cDNA library; Cell wall degrading enzymes; GABA-shunt; Transporters; Redox balance Abstract: Fusarium graminearum is a phytopathogenic filamentous fungus attacking a wide range of plants including Humulus lupulus (hop). Transcriptional analysis of F. graminearum grown on minimal media containing hop cell wall or glucose as the sole carbon source was performed by applying a highly stringent method combining microarrays and a subtracted cDNA library. In addition to genes coding for various cell wall degrading enzymes (CWDE), several metabolic pathways were induced in response to the plant cell wall substrate. Many genes participating in these pathways are probably involved in cellular transport. But the most interesting was that all the genes composing the 4-aminobutyrate-shunt (GABA-shunt) were also up-regulated in the presence of plant cell wall material and were present in the cDNA library. This study provides a description of a part of the fungal gene expression profile when it is in contact with raw biological materials, and helps in understanding the plant cell wall degradation and the infection process. Author Affiliation: (a) U.M.R. 7175, Universite Louis Pasteur-CNRS, Ecole Superieure de Biotechnologie de Strasbourg, Boulevard Sebastien Brant, BP 10413, 67412 Illkirch-Graffenstaden, France (b) Biogemma S.A.S., 24 Avenue des Landais, Les Cezeaux, 63170 Aubiere, France (c) febit biotech GmbH, Im Neuhenheimer Feld 519, 69120 Heidelberg, Germany (d) Institut de Medecine Legale, Universite Louis Pasteur, 11 rue Humann 67085 Strasbourg, France Article History: Received 14 September 2007; Accepted 3 December 2007