학술논문
Structural and kinetic characterization of active-site histidine as a proton shuttle in catalysis by human carbonic anhydrase II
Document Type
Academic Journal
Author
Source
Biochemistry. Feb 1, 2005, Vol. 44 Issue 4, 1097-1105
Subject
Language
English
ISSN
0006-2960
Abstract
The catalysis and crystal structures of wild-type human carbonic anhydrase II (HCA II) and two double mutants, H64A/N62H and H64A/N67H HCA II are examined to evaluate the effect of the position of the shuttle histidine and pH on proton shuttling. The observations in the crystal structures of a completed hydrogen-bonded water chain between the histidine shuttle residue and the zinc-bound solvent does not appear to be required for efficient proton transfer.