부산대학교 도서관

Transcriptomics has become an important tool for the large-scale analysis of biological processes. This review aims to provide sufficient criteria to make an appropriate choice among the variety of 'closed' systems, represented by DNA microarrays, and 'open' systems like fragment display, tag sequencing and subtractive hybridization, depending on the biological system under investigation. The most important technologies currently available are presented, their strengths and weaknesses are discussed and companies active in the field are listed. The potential of transcriptomics in the pharmaceutical research and development process is highlighted by applications in oncology, research on neurological diseases, and predictive toxicology. Finally, a prognosis for future developments of the technologies is given. Keywords: DNA arrays, fragment display, gene expression, MPSS, neurological diseases, oncology, RMDD, SAGe, toxicology, transcriptomics
Introduction The large-scale view of mRNAs transcribed from a cell's genome has been termed transcriptomics. Transcriptomics includes a variety of experimental approaches that have become an important toolbox to gain [...]