부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.mimet.2005.06.015 Byline: Maria Guirola (a), Tania Carmenate (b), Tamara Menendez (a), Anabel Alvarez (a), Sonia Gonzalez (a), Gerardo Guillen (a) Keywords: Capsular polysaccharide; ELISA; Immune response; Neisseria meningitidis Abstract: In this study we compared the following ELISA protocols to measure antibody levels against serogroup C meningococcal polysaccharide: a traditional protocol using poly-l-Lysine mixed with the polysaccharide as coating antigen, a second protocol coating with a mixture of methylated human serum albumin with the C polysaccharide, a modified protocol coating with derivatized polysaccharide and a modification to the last one, specifically without adding ammonium thiocyanate to the sample buffer. Serum bactericidal activity of mouse, monkey and human sera were measured and correlation coefficients were calculated. For all serum types the modified ELISA protocol showed the highest correlation coefficients while the traditional protocol showed the lower ones. We demonstrated that the traditional protocol measures non-specific antibodies to the C polysaccharide, because no differences were detected between pre-immune and post-immune human sera (P 0.05), while the modified protocol detected the highest difference (P Author Affiliation: (a) Division de Vacunas, Centro de Ingenieria Genetica y Biotecnologia, Ciudad de La Habana, Cuba (b) Laboratorio de Antigenos Sinteticos, Universidad de La Habana, Ciudad de La Habana, Cuba Article History: Received 28 January 2005; Revised 28 June 2005; Accepted 30 June 2005