학술논문
Organotypic culture assays for murine and human primary and metastatic-site tumors
Document Type
Report
Author
Source
Nature Protocols. August 2020, Vol. 15 Issue 8, p2413, 30 p.
Subject
Language
English
ISSN
1754-2189
Abstract
Author(s): Veena Padmanaban [sup.1] , Eloise M. Grasset [sup.1] , Neil M. Neumann [sup.1] , Andrew K. Fraser [sup.1] , Elodie Henriet [sup.1] , William Matsui [sup.2] [sup.5] , Phuoc [...]
Cancer invasion and metastasis are challenging to study in vivo since they occur deep inside the body over extended time periods. Organotypic 3D culture of fresh tumor tissue enables convenient real-time imaging, genetic and microenvironmental manipulation and molecular analysis. Here, we provide detailed protocols to isolate and culture heterogenous organoids from murine and human primary and metastatic site tumors. The time required to isolate organoids can vary based on the tissue and organ type but typically takes This protocol describes the isolation and 3D culture of organoids from fresh murine or human primary and metastatic tumor tissue and provides instructions for real-time imaging, genetic and microenvironmental manipulation and molecular analysis.
Cancer invasion and metastasis are challenging to study in vivo since they occur deep inside the body over extended time periods. Organotypic 3D culture of fresh tumor tissue enables convenient real-time imaging, genetic and microenvironmental manipulation and molecular analysis. Here, we provide detailed protocols to isolate and culture heterogenous organoids from murine and human primary and metastatic site tumors. The time required to isolate organoids can vary based on the tissue and organ type but typically takes This protocol describes the isolation and 3D culture of organoids from fresh murine or human primary and metastatic tumor tissue and provides instructions for real-time imaging, genetic and microenvironmental manipulation and molecular analysis.