학술논문
Evaluation of the AusDiagnostics 16-well Viral and Syphilis panel for use at SCL Dunedin
Otago 4th Year BMLSc Student Research Project Abstracts: Semester 2, 2022
Otago 4th Year BMLSc Student Research Project Abstracts: Semester 2, 2022
Document Type
Academic Journal
Author
Source
New Zealand Journal of Medical Laboratory Science. July 2023, Vol. 77 Issue 2, p97, 1 p.
Subject
Language
English
ISSN
1171-0195
Abstract
Jordan Oxenham (1,2) and Jenny Grant (2) (1) University of Otago, Dunedin and (2) Southern Community Laboratories, [...]
Objectives: Recently, there has been a worldwide monkeypox outbreak. In response, AusDiagnostics has developed a 16-well Viral and Syphilis PCR panel which includes monkeypox and other similar-presenting pathogens, such as syphilis and enterovirus. The aim of this study was to verify and validate targets of interest on this panel and validate extraction-free testing to evaluate the feasibility of using this panel at SCL Dunedin. Methods: Forty-four samples were selected to detect targets of interest; of particular interest were samples of sexually-transmitted infections. Each sample was run extracted and unextracted. Samples were extracted on the MT-Prep. Paired unextracted samples were run using the Viral and Syphilis panel on the High-Plex, a multiplex tandem PCR system. Results of extracted and unextracted runs were compared to diagnostic testing to assess concordance. Results: There was strong concordance between diagnostic testing and extracted samples on the 16-well panel. This concordance was less when comparing diagnostic testing and unextracted samples, mostly because all known syphilis samples had an inhibited SPIKE sequence. Take-off values were generally lower for extracted samples compared to diagnostic testing and unextracted samples. However, the viral panel currently used for diagnostic testing is run unextracted, and diagnostic testing and unextracted samples had similartake-offvalues. Conclusion: Our findings highlighted the advantages of using syndromic panels in testing for monkeypox, as known monkeypox-negative samples returned positive results for enterovirus and syphilis. While the targets were able to be verified and validated, we were unable to establish whether extraction-free testing could be performed. Furthertesting on the effect of universal transport media on SPIKE inhibition, particularly on syphilis samples, is planned to assess this suitability.
Objectives: Recently, there has been a worldwide monkeypox outbreak. In response, AusDiagnostics has developed a 16-well Viral and Syphilis PCR panel which includes monkeypox and other similar-presenting pathogens, such as syphilis and enterovirus. The aim of this study was to verify and validate targets of interest on this panel and validate extraction-free testing to evaluate the feasibility of using this panel at SCL Dunedin. Methods: Forty-four samples were selected to detect targets of interest; of particular interest were samples of sexually-transmitted infections. Each sample was run extracted and unextracted. Samples were extracted on the MT-Prep. Paired unextracted samples were run using the Viral and Syphilis panel on the High-Plex, a multiplex tandem PCR system. Results of extracted and unextracted runs were compared to diagnostic testing to assess concordance. Results: There was strong concordance between diagnostic testing and extracted samples on the 16-well panel. This concordance was less when comparing diagnostic testing and unextracted samples, mostly because all known syphilis samples had an inhibited SPIKE sequence. Take-off values were generally lower for extracted samples compared to diagnostic testing and unextracted samples. However, the viral panel currently used for diagnostic testing is run unextracted, and diagnostic testing and unextracted samples had similartake-offvalues. Conclusion: Our findings highlighted the advantages of using syndromic panels in testing for monkeypox, as known monkeypox-negative samples returned positive results for enterovirus and syphilis. While the targets were able to be verified and validated, we were unable to establish whether extraction-free testing could be performed. Furthertesting on the effect of universal transport media on SPIKE inhibition, particularly on syphilis samples, is planned to assess this suitability.