학술논문
Applicability of the critical chromatography concept to proteomic problems. II. Effect of mobile phase on the separation of peptides and proteins taking into account the amino acid sequence
Document Type
Report
Author
Source
Journal of Analytical Chemistry. December 2016, Vol. 71 Issue 12, p1188, 10 p.
Subject
Language
English
ISSN
1061-9348
Abstract
The effect of parameters of a binary mobile phase (chemical properties of components, gradient profile, flow rate, and pH) on the separation of peptides and proteins is theoretically investigated using a model of critical chromatography (model BioLCCC). It is demonstrated that the retention volume of peptides can be inverted by changing separation conditions. Keywords: high performance liquid chromatography, proteins and peptides, critical chromatography of biopolymers
The BioLCCC model of critical chromatography satisfactorily describes the nature of the macromolecules separation with different sequences, including peptides and proteins [1-8]. This model combines both microscopic parameters, such as [...]
The BioLCCC model of critical chromatography satisfactorily describes the nature of the macromolecules separation with different sequences, including peptides and proteins [1-8]. This model combines both microscopic parameters, such as [...]