학술논문
Characterization of monoclonal antibodies to the plasma cell alloantigen ENPP1
Document Type
Report
Author
Source
Hybridoma. February 1, 2011, Vol. 30 Issue 1, p11, 7 p.
Subject
Language
English
ISSN
1554-0014
Abstract
Introduction THE ECTONUCLEOTIDE PYROPHOSPHATASE PHOSPHODIESTERASE 1 (ENPP1) was discovered in 1970 by Takahashi, Old, and Boyse as a membrane alloantigen expressed by neoplastic plasma cells (PCs), termed plasmacytomas, and normal [...]
The ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) has documented roles in mineralization, nucleotide recycling, and insulin resistance. While ENPP1 was first identified as an alloantigen on mouse plasma cells (PCs), later studies revealed expression in many tissues. Previously described monoclonal antibodies against ENPP1 expressed at the cell surface recognized cells only from mice bearing the a allotype, ENPP1a, precluding studies of mice bearing the alternative allele, ENPP1b. Here, we characterize a novel anti-ENPP1 monoclonal antibody that recognizes both alleles and can be used for flow cytometry.
The ectonucleotide pyrophosphatase phosphodiesterase 1 (ENPP1) has documented roles in mineralization, nucleotide recycling, and insulin resistance. While ENPP1 was first identified as an alloantigen on mouse plasma cells (PCs), later studies revealed expression in many tissues. Previously described monoclonal antibodies against ENPP1 expressed at the cell surface recognized cells only from mice bearing the a allotype, ENPP1a, precluding studies of mice bearing the alternative allele, ENPP1b. Here, we characterize a novel anti-ENPP1 monoclonal antibody that recognizes both alleles and can be used for flow cytometry.