학술논문
Microbial fermentation of Fossence[TM], a short-chain fructo-oligosaccharide, under simulated human proximal colonic condition and assessment of its prebiotic effects-a pilot study
RESEARCH LETTER-Food Microbiology
RESEARCH LETTER-Food Microbiology
Document Type
Report
Author
Source
FEMS Microbiology Letters. Nov-Dec 2021, Vol. 368 Issue 24, p1b, 13 p.
Subject
Language
English
ISSN
0378-1097
Abstract
INTRODUCTION It is widely established that non-digestible oligosaccharides (NDOs), when consumed, are fermented in the human intestine by gut-microbiota (Sabater-Molina et al. 2009; Roberfroid et al. 2010; Dominguez et al. [...]
A short-chain fructo-oligosaccharide (sc-FOS) was tested in a simulator of the human gut microbial ecosystem (SHIME) in vitro model to quantify its prebiotic effects according to Prebiotic Index (PI) and Measure of prebiotic effect (MPE) equations. Fossence[TM], (sc-FOS, 0.5%) was fermented in a simulated human proximal colonic condition, using a fecal inoculum from a healthy individual. We analysed the pH reduction, substrate utilization, lactate and short-chain fatty acid (SCFA) production and microbial community modulation. Microbial fermentation of sc-FOS strongly reduced the media pH indicating the production of lactate and SCFA with accumulation of lactate and enhanced levels of acetate (34.38 [+ or -] 0.38 mM), propionate (20.93 [+ or -] 0.56 mM) and butyrate (4.93 [+ or -] 0.03 mM) compared to 18.46 [+ or -] 0.20 mM, 6.24 [+ or -] 0.10 mM and 3.3 [+ or -] 0.06 mM in the blank, respectively. Total SCFA production in test media was 61.91 [+ or -] 0.87 mM compared to 33.65 [+ or -] 0.36 mM in blank and the contribution of free-sugars present in sc-FOS to SCFAs was negligible. Modulation of the microbial community was analysed through 16S rRNA sequencing and we found that sc-FOS greatly stimulated the beneficial bacteria such as Bifidobacteria and Lactobacillus. We report the PI and MPE values for Fossence[TM], as 14.9 and 0.01 respectively at the end of 24 h, which is an indicator of a strong prebiotic effect. Keywords: sc-FOS; Prebiotic Index; inulin-type fructan; Fossence (TM);measureofprebioticindex;SCFA
A short-chain fructo-oligosaccharide (sc-FOS) was tested in a simulator of the human gut microbial ecosystem (SHIME) in vitro model to quantify its prebiotic effects according to Prebiotic Index (PI) and Measure of prebiotic effect (MPE) equations. Fossence[TM], (sc-FOS, 0.5%) was fermented in a simulated human proximal colonic condition, using a fecal inoculum from a healthy individual. We analysed the pH reduction, substrate utilization, lactate and short-chain fatty acid (SCFA) production and microbial community modulation. Microbial fermentation of sc-FOS strongly reduced the media pH indicating the production of lactate and SCFA with accumulation of lactate and enhanced levels of acetate (34.38 [+ or -] 0.38 mM), propionate (20.93 [+ or -] 0.56 mM) and butyrate (4.93 [+ or -] 0.03 mM) compared to 18.46 [+ or -] 0.20 mM, 6.24 [+ or -] 0.10 mM and 3.3 [+ or -] 0.06 mM in the blank, respectively. Total SCFA production in test media was 61.91 [+ or -] 0.87 mM compared to 33.65 [+ or -] 0.36 mM in blank and the contribution of free-sugars present in sc-FOS to SCFAs was negligible. Modulation of the microbial community was analysed through 16S rRNA sequencing and we found that sc-FOS greatly stimulated the beneficial bacteria such as Bifidobacteria and Lactobacillus. We report the PI and MPE values for Fossence[TM], as 14.9 and 0.01 respectively at the end of 24 h, which is an indicator of a strong prebiotic effect. Keywords: sc-FOS; Prebiotic Index; inulin-type fructan; Fossence (TM);measureofprebioticindex;SCFA