부산대학교 도서관

Byline: Jennifer Rodger (1), Douglas O. Frost (2) Keywords: Lateral geniculate nucleus; Superior colliculus; Neurotrophin; Visual system Abstract: TrkB is an important receptor for brain-derived neurotrophic factor and NT4, members of the neurotrophin family. TrkB signaling is crucial in many activity-dependent and activity-independent processes of neural development. Here, we investigate the role of trkB signaling in the development of two distinct, organizational features of retinal projections--the segregation of crossed and uncrossed retinal inputs along the "lines of projection" that represent a single point in the visual field and the "retinotopic" mapping of retinofugal axons within their cerebral targets. Using anterograde tracing, we obtained quantitative measures of the distribution of retinal projections in the dorsal nucleus of the lateral geniculate body (LGd) and superior colliculus (SC) of wild-type mice and mice homozygous for constitutive null mutation (knockout) of the full-length trkB receptor (trkB .sub.FL .sup.-/- ). In trkB .sub.FL .sup.-/- mice, uncrossed retinal projections cluster normally but there is a topographic expansion in the distribution of these clusters across the SC. By contrast, the absence of trkB signaling has no significant effect on the segregation of crossed and uncrossed retinal projections along the lines of projection in LGd or SC. We conclude that the normal topographic organization of uncrossed retinal projections depends upon trkB signaling, whereas the segregation of crossed and uncrossed retinal projections is trkB-independent. We also found that in trkB .sub.FL .sup.-/- mice, neuronal number was reduced in the LGd and SC and in the caudate-putamen. Previous studies by ourselves and others have shown that the number of retinal ganglion cells (RGCs) is unchanged in trkB .sub.FL .sup.-/- mice. Together, these results demonstrate that there is no matching of the numbers of RGCs with neuronal numbers in the LGd or SC. Author Affiliation: (1) Experimental and Regenerative Neurosciences, School of Animal Biology M317, University of Western Australia, 35 Stirling Highway, Crawley, WA, 6009, Australia (2) Program in Neuroscience, Department of Pharmacology and Experimental Therapeutics, University of Maryland School of Medicine, 655 West Baltimore St., Baltimore, MD, 21201, USA Article History: Registration Date: 17/02/2009 Received Date: 15/05/2008 Accepted Date: 16/02/2009 Online Date: 13/03/2009