학술논문
Serotonin transporter oligomerization documented in RN46A cells and neurons by sensitized acceptor emission FRET and fluorescence lifetime imaging microscopy
Document Type
Report
Author
Source
Biochemical and Biophysical Research Communications. March 20, 2009, Vol. 380 Issue 4, p724, 5 p.
Subject
Language
English
ISSN
0006-291X
Abstract
To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.bbrc.2009.01.128 Byline: Anja W. Fjorback (a)(b), Patrick Pla (c)(d), Heidi K. Muller (b), Ove Wiborg (b), Frederic Saudou (c)(d), Jens R. Nyengaard (a) Keywords: SERT; Monoamine transporter; Hippocampal neurons; RN46A cells; FRET and FLIM Abstract: The serotonin transporter is a member of the monoamine transporter family that also includes transporters of dopamine and norepinephrine. We have used sensitized acceptor emission fluorescence resonance energy transfer (FRET) and fluorescence lifetime imaging microscopy (FLIM) to study the oligomerization of SERT in HEK-MSR-239 cells, RN46A cells and in cultured hippocampal neurons. We were able to show identical FRET efficiencies in cell lines as well as in primary cultured hippocampal neurons, demonstrating that the oligomerization is cell type independent. The results obtained with both FRET approaches are very similar and furthermore, in agreement with previous results obtained by donor bleaching FRET microscopy. Author Affiliation: (a) Stereology and Electron Microscopy Research Laboratory, HistoInformatics and MIND Center, University of Aarhus, Ole Worms Alle, Building 8, DK-8000 Aarhus C, Denmark (b) Centre for Psychiatry Research, University Hospital Aarhus, Skovagervej 2, DK-8240 Risskov, Denmark (c) Institute Curie, Orsay F-91405, France (d) CNRS UMR146, Orsay F-91405, France Article History: Received 7 January 2009