학술논문

Assessment of genetic diversity in some Iranian Landraces of bread wheat (Triticum aestivum) using inter simple sequence repeat markers
Document Type
Report
Source
Advances in Environmental Biology. December 1, 2013, p4719, 5 p.
Subject
Iran
Language
English
ISSN
1995-0756
Abstract
The present study was conducted to evaluate the genetic diversity within a set of twenty Iranian landraces of bread wheat (Triticumaestivum) using inter simple sequence repeats (ISSR). Fifteen ISSR primers were used to estimate genetic diversity among genotypes. The primers produced 178 bands, among which 138 bands (about 77%) were polymorphic. A total of 138 polymorphic fragments were scored with average 9.7 polymorphic bands per primer. Cluster analysis using UPGMA method and Dice similarity coefficient categorized the genotypes into two main groups. Group one consisted of two genotypes and the other genotypes were arranged in the second group. Estimate of genetic similarity based on 138 polymorphic bands ranged from 0.35 to 0.83 with an average of 0.61. The presence of genetic diversity among the accessions provides important information for management of germplasm resources, genetic analysis and mapping QTLs with regard to future domestication and breeding programs. Key words: Bread Wheat, Genetic Diversity, ISSR, Landraces
INTRODUCTION Genetic diversity in cultivated crops such as wheat (TriticumaestivumL.) is essential for successful breeding and creation of new cultivars [2]. Because of the limited parent germplasm resources in breeding, [...]