학술논문
Year-round dynamics of amplicon sequence variant communities differ among eukaryotes, Imiteruirales and prokaryotes in a coastal ecosystem
RESEARCH ARTICLE
RESEARCH ARTICLE
Document Type
Report
Author
Source
FEMS Microbiology Ecology. December 2021, Vol. 97 Issue 12, p1r, 15 p.
Subject
Language
English
ISSN
0168-6496
Abstract
INTRODUCTION Microorganisms and viruses are largely responsible for biogeochemical cycling in marine ecosystems (Not et al. 2012; Brum and Sullivan 2015; Fuhrman, Cram and Needham 2015). The compositions of microbial [...]
Coastal microbial communities are affected by seasonal environmental change, biotic interactions and fluctuating nutrient availability. We investigated the seasonal dynamics of communities of eukaryotes, a major group of double-stranded DNA viruses that infect eukaryotes (order Imiteruirales; phylum Nudeocytouiricota), and prokaryotes in the Uranouchi Inlet, Kochi, Japan. We performed metabarcoding using ribosomal RNA genes and viral poIB genes as markers in 43 seawater samples collected over 20 months. Eukaryotes, prokaryotes and Imiteruirales communities characterized by the compositions of amplicon sequence variants (ASVs) showed synchronic seasonal cycles. However, the community dynamics showed intriguing differences in several aspects, such as the recovery rate after a year. We also showed that the differences in community dynamics were at least partially explained by differences in recurrence/persistence levels of individual ASVs among eukaryotes, prokaryotes and Imiteruirales. Prokaryotic ASVs were the most persistent, followed by eukaryotic ASVs and Imiteruirales ASVs, which were the least persistent. We argue that the differences in the specificity of interactions (virus-eukaryote vs prokaryote-eukaryote) as well as the niche breadth of community members were at the origin of the distinct community dynamics among eukaryotes, their viruses and prokaryotes. Keywords: Mimiuiridae; community dynamics; 16S; 18S; coastal seawater; seasonal dynamics
Coastal microbial communities are affected by seasonal environmental change, biotic interactions and fluctuating nutrient availability. We investigated the seasonal dynamics of communities of eukaryotes, a major group of double-stranded DNA viruses that infect eukaryotes (order Imiteruirales; phylum Nudeocytouiricota), and prokaryotes in the Uranouchi Inlet, Kochi, Japan. We performed metabarcoding using ribosomal RNA genes and viral poIB genes as markers in 43 seawater samples collected over 20 months. Eukaryotes, prokaryotes and Imiteruirales communities characterized by the compositions of amplicon sequence variants (ASVs) showed synchronic seasonal cycles. However, the community dynamics showed intriguing differences in several aspects, such as the recovery rate after a year. We also showed that the differences in community dynamics were at least partially explained by differences in recurrence/persistence levels of individual ASVs among eukaryotes, prokaryotes and Imiteruirales. Prokaryotic ASVs were the most persistent, followed by eukaryotic ASVs and Imiteruirales ASVs, which were the least persistent. We argue that the differences in the specificity of interactions (virus-eukaryote vs prokaryote-eukaryote) as well as the niche breadth of community members were at the origin of the distinct community dynamics among eukaryotes, their viruses and prokaryotes. Keywords: Mimiuiridae; community dynamics; 16S; 18S; coastal seawater; seasonal dynamics