부산대학교 도서관

To purchase or authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1365-2990.2006.00722.x Byline: E. Croisier (*), D. Elfant (*), M. Deprez ([double dagger]), K. Goldring ([dagger]), D. T. Dexter ([dagger]), R. K. B. Pearce (*), M. B. Graeber (*), F. Roncaroli (*) Keywords: alpha-synuclein; immunohistochemistry Abstract: Immunohistochemistry for alpha-synuclein has become the histological technique of choice for the diagnosis for Parkinson's disease, Dementia with Lewy bodies and Multiple System Atrophy (http://www.ICDNS.org). Nevertheless, no standardised protocol has been proposed. We have reviewed 242 of the 270 studies published until June 2005 that mentioned immunohistochemistry for anti-alpha synuclein on human tissue and we found that only 75 (31%) used commercial antibodies. We also noted that protocols, particularly dilution and antigen unmasking, varied between studies, even when the same antibody was employed. In order to establish a standardised protocol for alpha-synuclein immunohistochemistry, which can be applied in diagnostic neuropathology we tested seven commercial monoclonal antibodies in brains of subjects with Parkinson's disease, dementia with Lewy bodies, multiple system atrophy, multiple sclerosis with incidental Lewy bodies and aged-matched normal brain and determined for each antibody the best suited protocol for antigen unmasking. We evaluated the intensity of immunolabelling in Lewy bodies, neuropil threads, dendrites, pre-synaptic terminals, granular cytoplasmic positivity, peri-axonal positivity, glial inclusions and non-specific immunolabelling. Although our results showed that all the antibodies detected alpha-synuclein inclusions, differences were noted between antibodies, particularly with regard to the detection of glial inclusions. From our study, the best antibodies of the seven tested appeared to be those directed against amino acids 116-131 and 15-123 and we suggest them to be used in routine diagnostic practice for alpha-synucleinopathies. Author Affiliation: (*)Department of Neuropathology, Imperial College London, ([dagger])UK Parkinson's Disease Society Tissue Bank, Department of Cellular and Molecular Neuroscience, Imperial College London, UK, and ([double dagger])Laboratory of Neuropathology, Department of Pathology, University of Liege, Belgium Article History: Received 1 July 2005 Accepted after revision 8 November 2005 Article note: Federico Roncaroli, University Department of Neuropathology, Division of Neuroscience and Mental Health, Imperial College London and Hammersmith Hospitals Trust, Charing Cross Campus, St Dunstan's Road, London W6 8RP, UK. Tel: +44 208 8467178; Fax: +44 020 88467794; E-mail: f.roncaroli@imperial.ac.uk