부산대학교 도서관

Escalating rates of obesity and public health messages to reduce excessive sugar intake have fuelled the consumption of artificial sweeteners (AS) in a wide range of products from breakfast cereals to snacks and beverages. Several studies indicate an association of AS consumption with increased appetite, food intake, weight gain and glucose intolerance. Multiple studies have studied the effect of AS on glucose absorption in the gut, there are very few probing the same in adipocytes. Therefore, we want to study the effect of different AS on glucose uptake in adipocytes. The aim is to study effect of saccharin, sucralose and cyclamate on glucose uptake in 3T3 cell lines. 3T3 cells (mouse origin) from ATCC (American Type Culture Collection) were grown in Dulbecco's Modified Eagle's Medium (DMEM). After differentiation, they were incubated overnight with the AS (0, 1 nmol, 1 mmol and 1 mmol of the 3 AS) and glucose uptake assay was performed the next day for basal and insulin stimulated uptake. All experiments were performed in triplicates. 2 way Anova was done. Almost all of them showed significant increase in uptake in stimulated condition compared to basal. Cyclamate and saccharin showed an increase in uptake but sucralose shows a significant decrease in uptake with increase in concentration. AS are usually consumed by people with insulin resistance. All AS are not having similar effect on glucose uptake by cells. Sucralose is decreasing the glucose uptake by cells and may not be the AS of choice in people who already have decreased glucose uptake. Whereas, Saccharin and cyclamate are increasing glucose uptake and could be beneficial in individuals with insulin resistance. AS are not metabolically inert and it will be necessary to choose the right AS based on the metabolic status of the individual.
P-305 Devanath A, Prakash S N, Shanthakumari J St John's Medical College, Bangalore, [...]