학술논문
MicroRNA-34a-5p expression in the plasma and in its extracellular vesicle fractions in subjects with Parkinson's disease: An exploratory study
Document Type
Academic Journal
Author
Source
International Journal of Molecular Medicine. February 2021, Vol. 47 Issue 2, p533, 14 p.
Subject
Language
English
ISSN
1107-3756
Abstract
Introduction Parkinson's disease (PD) is the second most common neurodegenerative disease worldwide affecting 1% of the population that is over 60 years of age. The main pathological feature is the [...]
Parkinson's disease (PD) is an important disabling age-related disorder and is the second most common neurodegenerative disease. currently, no established molecular biomarkers exist for the early diagnosis of PD. circulating microRNAs (miRNAs), either vesicle-free or encapsulated in extracellular vesicles (EVs), have emerged as potential blood-based biomarkers also for neurodegenerative diseases. In this exploratory study, we focused on miR-34a-5p because of its well-documented involvement in neurobiology. To explore a differential profile of circulating miR-34a-5p in PD, PD patients and age-matched control subjects were enrolled. Serial ultracentrifugation steps and density gradient were used to separate EV subpopulations from plasma according to their different sedimentation properties (Large, Medium, Small EVs). characterization of EV types was performed using western blotting and atomic force microscopy (AFM); purity from protein contaminants was checked with the colorimetric nanoplasmonic assay. circulating miR-34a-5p levels were evaluated using qPCR in plasma and in each EV type. miR-34a-5p was significantly up-regulated in small EVs devoid of exogenous protein contaminants (pure SEVs) from PD patients and ROC analysis indicated a good diagnostic performance in discriminating patients from controls (AUc=0.74, P Key words: Parkinson's disease, human plasma, extracellular vesicles, microRNAs, miR-34a-5p
Parkinson's disease (PD) is an important disabling age-related disorder and is the second most common neurodegenerative disease. currently, no established molecular biomarkers exist for the early diagnosis of PD. circulating microRNAs (miRNAs), either vesicle-free or encapsulated in extracellular vesicles (EVs), have emerged as potential blood-based biomarkers also for neurodegenerative diseases. In this exploratory study, we focused on miR-34a-5p because of its well-documented involvement in neurobiology. To explore a differential profile of circulating miR-34a-5p in PD, PD patients and age-matched control subjects were enrolled. Serial ultracentrifugation steps and density gradient were used to separate EV subpopulations from plasma according to their different sedimentation properties (Large, Medium, Small EVs). characterization of EV types was performed using western blotting and atomic force microscopy (AFM); purity from protein contaminants was checked with the colorimetric nanoplasmonic assay. circulating miR-34a-5p levels were evaluated using qPCR in plasma and in each EV type. miR-34a-5p was significantly up-regulated in small EVs devoid of exogenous protein contaminants (pure SEVs) from PD patients and ROC analysis indicated a good diagnostic performance in discriminating patients from controls (AUc=0.74, P Key words: Parkinson's disease, human plasma, extracellular vesicles, microRNAs, miR-34a-5p