부산대학교 도서관

To authenticate to the full-text of this article, please visit this link: http://dx.doi.org/10.1111/j.1550-7408.2009.00390.x Byline: MANUELA BAUMGARTNER (a), SILVIA EBERHARDT (a), JOHAN F. DE JONCKHEERE (b), KARL O. STETTER (a) Keywords: Acidophilic; ITS; monoxenic culture; SSU rRNA; thermophilic; Vahlkampfiidae Abstract: ABSTRACT. Tetramitus thermacidophilus n. sp. is a novel thermophilic and acidophilic amoeboflagellate isolated from acidic hot springs in the Caldera Uzon (Kamchatka, Russia) and in Pisciarelli Solfatara (Naples, Italy). We describe it based on physiological, morphological, and sequence data. It was grown in monoxenic culture on the archaeon Acidianus brierleyi as food. Tetramitus thermacidophilus multiplies in a pH range from 1.2 to 5 and in a temperature range from 28 [degrees]C to 54 [degrees]C. The shortest doubling time was 4.5 h at pH 3 at 45 [degrees]C. Its spindle-shaped biflagellated stage was only rarely found in culture. The amoeboid stage shows the typical locomotive form of vahlkampfiid amoebae. Sequence comparisons of the internal transcribed spacer sequences and the small subunit rRNA genes confirm that T. thermacidophilus is a novel species within the genus Tetramitus and that both isolates belong to that species. Author Affiliation: (a)Lehrstuhl fur Mikrobiologie, Universitat Regensburg, D-93053 Regensburg, Germany (b)Scientific Institute of Public Health, B-1050 Brussels, Belgium Article History: Received: 04/30/08, 09/09/08; accepted: 09/10/08 Article note: Corresponding Author: Karl O. Stetter, Lehrstuhl fur Mikrobiologie, Universitat Regensburg, Universitatsstrasse 31, D-93053 Regensburg, Germany-Telephone number: +49 (0)89 68 096 548; Fax number: +49 (0)89 68 096 580; e-mail: karl.stetter@biologie.uni-regensburg.de