부산대학교 도서관

Purpose: We performed comparative proteomic analyses of blood of patients with RLS and healthy individuals aiming to identify potential biomarker and therapeutic target candidate for RLS. Patients and Methods: Blood serum samples from 12 patients with a clinical diagnosis of RLS (8 females and 4 males, with a mean age of 68.52 years) and 10 healthy controls (5 females and 5 males, with a mean age of 67.61 years) underwent proteomic profiling by liquid chromatography coupled with tandem mass spectrometry. Pathway analysis incorporating protein-protein interaction networks was carried out to identify pathological processes linked to the differentially expressed proteins. Results: We quantified 272 proteins in patients with RLS and healthy controls, of which 243 were shared. Five proteins--apolipoprotein C-II, leucine-rich alpha-2-glycoprotein 1, FLJ92374, extracellular matrix protein 1, and FLJ93143--were substantially increased in RLS patients, whereas nine proteins--vitamin D-binding protein, FLJ78071, alpha-1-antitrypsin, CD5 antigenlike, haptoglobin, fibrinogen alpha chain, complement factor H-related protein 1, platelet factor 4, and plasma protease C1 inhibitor--were decreased. Bioinformatics analyses revealed that these proteins were linked to 1) inflammatory and immune response, and complement activation, 2) brain-related development, cell aging, and memory disorders, 3) pregnancy and associated complications, 4) myocardial infarction, and 5) reactive oxygen species generation and subsequent diabetes mellitus. Conclusion: Our findings shed light on the multifactorial nature of RLS and identified a set of circulating proteins that may have clinical importance as biomarkers and therapeutic targets. Keywords: idiopathic restless legs syndrome, biomarkers, LC-MS/MS, proteome, interactome
Introduction Restless leg syndrome (RLS) (1) is a relatively common neurologic sleep-related movement disorder, with a reported prevalence ranging from 5% to 10% in the general population, making it the [...]