학술논문
Mistletoe (Viscum album L.) extract attenuates itraconazole-induced acute oxidative stress and hepatocellular injury in rats
Document Type
Report
Source
Pakistan Journal of Pharmaceutical Sciences. January 2023, Vol. 36 Issue 1, p9, 8 p.
Subject
Language
English
ISSN
1011-601X
Abstract
INTRODUCTION Itraconazole (ITZ) is a synthetic triazole with broad-spectrum antifungal activity. The key component of fungal cell membranes and the underlying molecular mechanism of ITZ is the suppression of the [...]
In the current study, the protective effect of a mistletoe extract (Helixor[R], HLX) on Itraconazole (ITZ)- induced hepatocellular injury and acute oxidative stress in rats was aimed to be investigated by histological, biochemical and comet assay methods. Four groups a control group, an HLX group (5mg/kg/14days/intraperitoneally (ip)), an ITZ group (100mg/kg/14days/oral) and an HLX plus ITZ group (5mg/kg/14days/ip+100mg/kg/14days/oral) were all created from 32 female Wistar albino rats. At the end of the experiment, AST and ALT liver enzymes, total oxidant status (TOS) levels and total antioxidant status (TAS) levels, histopathological analysis and comet assay were carried out. Highest genotoxicity, higher levels of plasma AST and ALT, higher TOS, more degeneration of liver histopathology including hepatocyte degeneration, hepatocyte apoptosis and necrosis, portal/periportal inflammation, bile ductus hyperplasia and multinuclear giant cell formation were observed in ITZ group (p Keywords: Itraconazole, helixor, oxidative stress, antioxidant activity, comet assay
In the current study, the protective effect of a mistletoe extract (Helixor[R], HLX) on Itraconazole (ITZ)- induced hepatocellular injury and acute oxidative stress in rats was aimed to be investigated by histological, biochemical and comet assay methods. Four groups a control group, an HLX group (5mg/kg/14days/intraperitoneally (ip)), an ITZ group (100mg/kg/14days/oral) and an HLX plus ITZ group (5mg/kg/14days/ip+100mg/kg/14days/oral) were all created from 32 female Wistar albino rats. At the end of the experiment, AST and ALT liver enzymes, total oxidant status (TOS) levels and total antioxidant status (TAS) levels, histopathological analysis and comet assay were carried out. Highest genotoxicity, higher levels of plasma AST and ALT, higher TOS, more degeneration of liver histopathology including hepatocyte degeneration, hepatocyte apoptosis and necrosis, portal/periportal inflammation, bile ductus hyperplasia and multinuclear giant cell formation were observed in ITZ group (p Keywords: Itraconazole, helixor, oxidative stress, antioxidant activity, comet assay