학술논문
Functional expression and production of human H-ferritin in Pichia pastoris
Document Type
Academic Journal
Author
Source
Biotechnology Letters. July, 2003, Vol. 25 Issue 13, p1019, 5 p.
Subject
Language
English
ISSN
0141-5492
Abstract
Human heavy chain ferritin (H-ferritin) was cloned from human heart cDNA library and expressed in Pichia pastoris. The H-ferritin transformant was cultivated by fed-batch and the cell mass reached about 52 g cell dry wt l.sup.-1 after 150 h. In atomic absorption spectrometry analysis, intracellular content of iron in H-ferritin transformant was measured to 3038 +- 72 ug g.sup.-1 which was 9.6-fold more than that of control strain.