부산대학교 도서관

To link to full-text access for this article, visit this link: http://dx.doi.org/10.1016/j.anireprosci.2005.02.007 Byline: Melissa L. Vadnais (a), Roy N. Kirkwood (a), David J. Specher (a), Karen Chou (b)(c) Keywords: Boar; Frozen; Spermatozoa; Seminal plasma; Capacitation Abstract: The effect on apparent capacitation status of frozen-thawed (FT), washed boar sperm was examined in capacitation-supporting medium at 39[degrees]C without added seminal plasma (SP) or supplemented with either 10 or 20% (v/v) boar SP. The thawed sperm from three boars were washed to remove the egg yolk-based freezing medium (EY) and then incubated for 1-8h after addition of SP. Capacitation status of the sperm was determined microscopically using chlortetracycline staining patterns. At 1h after the addition of 10 or 20% (v/v) SP, capacitated sperm decreased from 59.7 to 30.3% and from 59.5 to 26.8%, respectively (P Author Affiliation: (a) Department of Large Animal Clinical Sciences, Michigan State University, College Vet Med, East Lansing, MI 48824-1314, USA (b) Department of Animal Science, Michigan State University, East Lansing, MI, USA (c) Institute for Environmental Toxicology, Michigan State University, East Lansing, MI, USA Article History: Received 19 January 2005; Accepted 23 February 2005